Mouse anti-Human, Rat N-Cadherin Monoclonal Antibody | anti-NCAD antibody

N-Cadherin (CDHN, NCAD, Neural Cadherin, CD325) (AP)

Cat. Num. AAA6248185

• Gene Names: CDH2; CDHN; NCAD; CD325; CDw325
• Reactivity: Human, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by protein G affinity chromatography from tissue culture supernatant.
• Synonyms: N-Cadherin; Monoclonal Antibody; N-Cadherin (CDHN; NCAD; Neural Cadherin; CD325) (AP); anti-NCAD antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 13A9
• Specificity: Recognizes human N-Cadherin. Species Crossreactivity: Rat
• Purity/Purification: Purified by protein G affinity chromatography from tissue culture supernatant.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-NCAD antibody: Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Conjugate: AP
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Immunohistochemistry (IHC)

(Formalin fixed, paraffin embedded human breast cancer biopsy stained with 215041 followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (low power))

Immunohistochemistry (IHC)

(Formalin fixed, paraffin embedded human breast cancer biopsy stained with 215041 followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (high power))

Product Categories/Family for anti-NCAD antibody

Antibodies; Cadherin

References

1. Wahl, J.K. 3rd et al. (2003) N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane. J. Biol. Chem. 278: 17269-17276. 2. Knudsen, K.A. et al. (1995) Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin. J. Cell Biol. 130: 67.77. 3. Machell, N.H. et al. (2000) Developmental expression and distribution of N- and E-cadherin in the rat ovary. Biol. Reprod. 63: 797-804. 4. Han, A.C. et al. (1997) Differential expression of N-cadherin in pleural mesotheliomas and E-cadherin in lung adenocarcinomas in formalin-fixed, paraffin-embedded tissues. Hum. Pathol. 28: 641-645. 5. Peralta Soler, A. et al. (1995) The differential expression of N-cadherin and E-cadherin distinguishes pleural mesotheliomas from lung adenocarcinomas. Hum. Pathol. 26: 1363-1369. 6. Van Aken, E.H. et al. (2002) Structure and function of the N-cadherin/catenin complex in retinoblastoma. Invest Ophthalmol Vis Sci. 43: 595-602. 7. Shintani, Y. et al. (2006) Phosphoinositide-3 kinase-Rac1-c-Jun NH2-terminal kinase signaling mediates collagen I-induced cell scattering and up-regulation of N-cadherin expression in mouse mammary epithelial cells. Mol Biol Cell. 17: 2963-75. 8. Sacco, P.A. et al. (1995) Identification of plakoglobin domains required for association with N-cadherin and alpha- catenin. J Biol Chem. 270: 20201-6. 9. Tian, G. et al. (2009) Clarin-1, encoded by the Usher Syndrome III causative gene, forms a membranous microdomain: possible role of clarin-1 in organizing the actin cytoskeleton. J Biol Chem. 284: 18980-93. 10. Theisen, C.S. et al. (2007) NHERF links the N-cadherin/catenin complex to the platelet-derived growth factor receptor to modulate the actin cytoskeleton and regulate cell motility. Mol Biol Cell. 18: 1220-32. 11. Nieman, M.T.et al. (1999) N-cadherin promotes motility in human breast cancer cells regardless of their E- cadherin expression. J. Cell, Biol. 147: 631-44. 12. Islam, S.et al. (1996) Expression of N-cadherin by human squamous carcinoma cells induces a scattered fibroblastic phenotype with disrupted cell-cell adhesion. J. Cell. Biol.135: 1643-54. 13. Peralta Soler, A. et al. (1999) P-cadherin expression in breast carcinoma indicates poor survival. Cancer. 86: 1263-72.

NCBI and Uniprot Product Information

• NCBI GI #: 14589889
• NCBI GeneID: 1000
• NCBI Accession #: NP_001783.2
• NCBI GenBank Nucleotide #: NM_001792.3
• UniProt Accession #: P19022; Q9Z1Y3
• Molecular Weight: 99,809 Da
• NCBI Official Full Name: cadherin-2 preproprotein
• NCBI Official Synonym Full Names: cadherin 2, type 1, N-cadherin (neuronal)
• NCBI Official Symbol: CDH2
• NCBI Official Synonym Symbols: CDHN; NCAD; CD325; CDw325
• NCBI Protein Information: cadherin-2; N-cadherin 1; neural cadherin; neural-cadherin; cadherin 2, N-cadherin (neuronal); calcium-dependent adhesion protein, neuronal
• UniProt Protein Name: Cadherin-2
• UniProt Gene Name: CDH2
• UniProt Synonym Gene Names: CDHN; NCAD; N-cadherin
• UniProt Entry Name: CADH2_HUMAN

NCBI Description

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq, Jul 2008]

Uniprot Description

CDH2: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. Interacts with CDCP1. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Interacts with PCDH8; this complex may also include TAOK2. The interaction with PCDH8 may lead to internalization through TAOK2/p38 MAPK pathway.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: cell-cell adherens junction; focal adhesion; lamellipodium; apical plasma membrane; integral to membrane; plasma membrane; synapse; intercellular junction; fascia adherens; catenin complex

Molecular Function: protein binding; gamma-catenin binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; alpha-catenin binding

Biological Process: heterophilic cell adhesion; intercellular junction assembly and maintenance; muscle cell differentiation; cell migration; glial cell differentiation; positive regulation of MAPKKK cascade; calcium-dependent cell-cell adhesion; blood vessel morphogenesis; positive regulation of muscle cell differentiation; striated muscle cell differentiation; cell adhesion; homophilic cell adhesion