Rabbit anti-Mouse Carnitine Palmitoyl Transferase 1, Muscle Polyclonal Antibody | anti-CPT1C antibody

Carnitine Palmitoyl Transferase 1, Muscle (CPT1-M)

Cat. Num. AAA624631

• Gene Names: CPT1C; CATL1; CPT1P; CPTIC; CPT1-B; CPTI-B; FLJ23809
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Carnitine Palmitoyl Transferase 1; Muscle; Polyclonal Antibody; Muscle (CPT1-M); Anti -Carnitine Palmitoyl Transferase 1; anti-CPT1C antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Carnitine Palmitoyl Transferase 1, Muscle (CPT1-M). No significant sequence homology is seen with LCPT-1, CPT-2 or any other protein.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA.

• Applicable Applications for anti-CPT1C antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:10,000-1:100,000. Coat plates with 50-100ng of C1385-48: Carnitine Palmitoyl Transferase 1, Muscle, Mouse, Control Peptide/well.; Western Blot: 1-10ug/ml (ECL).
• Immunogen: Synthetic peptide corresponding to 13aa near the C-terminus of mouse CPT1-M conjugated to KLH. Species Sequence Homology: rat - 92%, human - 69%.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-CPT1C antibody

In cells and organisms, the excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA. In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1). Activities of ACC-1 and ACC-2 are regulated by their phosphorylation by 5'-AMP-activated protein kinase (AMPK). Diabetes deranges AMPK master-switch, represses the ACC-1 gene-expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. Cytosolic LCFA-CoA is converted into acyl-carnitine at mitochondrial membrane surface by carnitine palmitoyl transferase-1 (CPT-1) followed by the reconversion of the latter into LCFA-CoA by CPT-2 in the mitochondrial matrix. Mitochondrial oxidation of LC-FCA is initiated by the sequential action of CPT-1, which is located in the outer membrane, and CPT-2, which is located in the inner membrane together with a carnitine-acylcarnitine translocase. CPT-1 liver or CPT1A or LCPT-1 (mouse 764aa, rat 773aa, human 773aa, ~88kD, chromosome 11q13) is malonyl-CoA-sensitive enzyme localized on the outer surface of mitochondrial 'contact sites'. It catalyzes the conversion of long-chain acyl-CoA into acyl-carnitine, committing the acyl moiety to intramitochondrial oxidation. It is predominantly expressed in kidney, liver and in trace amounts in heart. The 'muscle' isoform CPT1B or CPT1M or MCPT-1 (mouse/rat/human 772aa, chromosome 22q13.3) is found in heart, skeletal muscle, adipose tissue and brain. The amino acid sequences of the two isoforms are ~61% identical.

Product Categories/Family for anti-CPT1C antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 209862905
• NCBI GeneID: 126129
• NCBI Accession #: NP_001129524.1
• NCBI GenBank Nucleotide #: NM_001136052.2
• UniProt Accession #: Q59H75; Q8TCG5
• Molecular Weight: 45,251 Da
• NCBI Official Full Name: carnitine O-palmitoyltransferase 1, brain isoform isoform 1
• NCBI Official Synonym Full Names: carnitine palmitoyltransferase 1C
• NCBI Official Symbol: CPT1C
• NCBI Official Synonym Symbols: CATL1; CPT1P; CPTIC; CPT1-B; CPTI-B; FLJ23809
• NCBI Protein Information: carnitine O-palmitoyltransferase 1, brain isoform; carnitine palmitoyltransferase I related C; carnitine O-palmitoyltransferase I, brain isoform
• UniProt Protein Name: Carnitine palmitoyltransferase 1C variant
• Protein Family: Carnitine O-palmitoyltransferase
• UniProt Entry Name: Q59H75_HUMAN

NCBI Description

This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein regulates the beta-oxidation and transport of long-chain fatty acids into mitochondria, and may play a role in the regulation of feeding behavior and whole-body energy homeostasis. Alternatively spliced transcript variants encoding multiple protein isoforms have been observed for this gene. [provided by RefSeq]

Uniprot Description

CPT1C: a member of the carnitine/choline acetyltransferase family. The encoded protein regulates the beta-oxidation and transport of long-chain fatty acids into mitochondria, and may play a role in the regulation of feeding behavior and whole-body energy homeostasis. Alternatively spliced transcript variants encoding multiple protein isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

Protein type: Membrane protein, integral; EC 2.3.1.21; Membrane protein, multi-pass; Lipid Metabolism - fatty acid; Transferase

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: mitochondrial outer membrane; endoplasmic reticulum membrane; synapse; cell junction

Molecular Function: carnitine O-palmitoyltransferase activity

Biological Process: fatty acid beta-oxidation; carnitine metabolic process

Disease: Spastic Paraplegia 73, Autosomal Dominant