Western Blot (WB)
(Peptide Inhibition: Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) (Lane 7) in Western Blot analysis of HeLa acid extracts and various other modified histone peptides.)
Mouse anti-Human Histone H3 Monoclonal Antibody | anti-HIST3H3 antibody
Histone H3, monomethyl (Lys9) (MaxLight 550)
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
Histone H3; Monoclonal Antibody; monomethyl (Lys9) (MaxLight 550); anti-HIST3H3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
10A19
Specificity
Recognizes human monomethylated Lys9 at and around the N-terminal of Histone H3, MW ~17kD. Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) in Western Blot analysis of HeLa acid extracts and various other modified histone peptides.
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Applicable Applications for anti-HIST3H3 antibody
Western Blot (WB)
Immunogen
Linear peptide corresponding to monomethylated Lys9 at the N-terminal region of human Histone H3, conjugated to KLH.
Conjugate
MaxLight550
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Western Blot (WB)
(Peptide Inhibition: Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) (Lane 7) in Western Blot analysis of HeLa acid extracts and various other modified histone peptides.)
Western Blot (WB)
(Peptide Inhibition: Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) (Lane 7) in Western Blot analysis of HeLa acid extracts and various other modified histone peptides.)
Western Blot (WB)
(Western Blot: Recombinant Histone H3 (Lane 1) and HeLa acid extract (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS608628 (1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.)
Western Blot (WB)
(Western Blot: Recombinant Histone H3 (Lane 1) and HeLa acid extract (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS608628 (1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.)
Product Categories/Family for anti-HIST3H3 antibody
References
1. Nakayama, J.I., et al., Science 292: 110-113 (2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,508 Da
NCBI Official Full Name
histone H3.1t
NCBI Official Synonym Full Names
histone cluster 3, H3
NCBI Official Symbol
HIST3H3
NCBI Official Synonym Symbols
H3t; H3.4; H3/g; H3FT
NCBI Protein Information
histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T
UniProt Protein Name
Histone-lysine N-methyltransferase SUV39H1
Protein Family
UniProt Gene Name
SUV39H1
UniProt Synonym Gene Names
KMT1A; SUV39H; H3-K9-HMTase 1
UniProt Entry Name
SUV91_HUMAN
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Product Notes
The HIST3H3 suv39h1 (Catalog #AAA6246206) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Histone H3, monomethyl (Lys9) (MaxLight 550) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the HIST3H3 suv39h1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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