Flow Cytometry (FC/FACS)
(Flow Cytometry Analysis: C57/BL6 mouse embryonic stem cells were labeled with the Oct-4 antibody at a 1:200 or 5ug dilution (Red) and a 1:500 or 2ug dilution (Green). The AlexaFluor®488 isotype control (1:1000) is shown in blue. (Representative lot data).)
Mouse anti-Human, Mouse 2019-10-04 Monoclonal Antibody | anti-OCT-4 antibody
OCT-4 (POU5F1, POU domain, class 5, transcription factor 1, NF-A3, Oct3, Oct-3, Oct3/4, Oct-3/4, Octamer-binding transcription factor 3, Otf3, Otf-3, Otf3g, Otf3-rs7, Otf4, Otf-4) (MaxLight 405)
Flow Cytometry (FC/FACS)
(Flow Cytometry Analysis: C57/BL6 mouse embryonic stem cells were labeled with the Oct-4 antibody at a 1:200 or 5ug dilution (Red) and a 1:500 or 2ug dilution (Green). The AlexaFluor®488 isotype control (1:1000) is shown in blue. (Representative lot data).)
Flow Cytometry (FC/FACS)
(Flow Cytometry Analysis: C57/BL6 mouse embryonic stem cells were labeled with the Oct-4 antibody at a 1:200 or 5ug dilution (Red) and a 1:500 or 2ug dilution (Green). The AlexaFluor®488 isotype control (1:1000) is shown in blue. (Representative lot data).)
Immunocytochemistry (ICC)
(Immunocytochemistry Analysis: C57/BL6 mouse embryonic stem cells labeled with DAPI (A) and 1:100 dilution of Oct-4 antibody (B). Human embryonic stem cells labeled with DAPI (C) and 1:200 dilution of Oct-4 antibody(D). (Representative lot data).)
Immunocytochemistry (ICC)
(Immunocytochemistry Analysis: C57/BL6 mouse embryonic stem cells labeled with DAPI (A) and 1:100 dilution of Oct-4 antibody (B). Human embryonic stem cells labeled with DAPI (C) and 1:200 dilution of Oct-4 antibody(D). (Representative lot data).)
Western Blot (WB)
(Western Blot Analysis: C57/BL6 mouse embryonic stem cell lysates were resolved by electrophoresis, transferred to PVDF, and probed with the Oct-4 antibody (1 ug/mL). Proteins were visualized using a conjugated goat anti-mouse secondary antibody and a chemiluminescence detection system. (Representative lot data).)
Western Blot (WB)
(Western Blot Analysis: C57/BL6 mouse embryonic stem cell lysates were resolved by electrophoresis, transferred to PVDF, and probed with the Oct-4 antibody (1 ug/mL). Proteins were visualized using a conjugated goat anti-mouse secondary antibody and a chemiluminescence detection system. (Representative lot data).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]
Uniprot Description
Oct4: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2. Transcriptional activity is positively regulated by PKM2. Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. Belongs to the POU transcription factor family. Class- 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; DNA-binding
Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleolus; nucleoplasm; nucleus; transcription factor complex; transcriptional repressor complex
Molecular Function: chromatin binding; chromatin DNA binding; cytokine binding; DNA binding; miRNA binding; protein binding; protein heterodimerization activity; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity; transcription factor binding; ubiquitin protein ligase binding
Biological Process: blastocyst growth; cell fate commitment; ectodermal cell fate commitment; endodermal cell fate commitment; endodermal cell fate specification; germ-line stem cell maintenance; mesodermal cell fate commitment; mRNA transcription from RNA polymerase II promoter; multicellular organismal development; negative regulation of calcium ion-dependent exocytosis; negative regulation of cell differentiation; negative regulation of protein kinase B signaling cascade; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of protein kinase B signaling cascade; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of gene expression; regulation of transcription, DNA-dependent; response to organic substance; response to retinoic acid; somatic stem cell maintenance; stem cell differentiation; stem cell maintenance; transcription from RNA polymerase II promoter; transcription, DNA-dependent; trophectodermal cell differentiation
Research Articles on OCT-4
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Product Notes
The OCT-4 pou5f1 (Catalog #AAA6245124) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The OCT-4 (POU5F1, POU domain, class 5, transcription factor 1, NF-A3, Oct3, Oct-3, Oct3/4, Oct-3/4, Octamer-binding transcription factor 3, Otf3, Otf-3, Otf3g, Otf3-rs7, Otf4, Otf-4) (MaxLight 405) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's 2019-10-04 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Western Blot (WB). Researchers should empirically determine the suitability of the OCT-4 pou5f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "2019-10-04, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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