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Western Blot (WB) (Western blot analysis of ARHGAP18 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). ARHGAP18 (arrow) was detected using the purified Pab.)

Rabbit anti-Human ARHGAP18 Polyclonal Antibody | anti-ARHGAP18 antibody

ARHGAP18, ID (Rho GTPase-activating Protein 18, Rho-type GTPase-activating Protein 18, MacGAP)

Gene Names
ARHGAP18; MacGAP; FLJ25728; MGC126757; MGC138145; bA307O14.2
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Synonyms
ARHGAP18; Polyclonal Antibody; ID (Rho GTPase-activating Protein 18; Rho-type GTPase-activating Protein 18; MacGAP); Anti -ARHGAP18; anti-ARHGAP18 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ARHGAP18.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-ARHGAP18 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunogen
Synthetic peptide selected from the Center region of human ARHGAP18 (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ARHGAP18 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). ARHGAP18 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of ARHGAP18 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). ARHGAP18 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with ARHGAP18 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human breast carcinoma reacted with ARHGAP18 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ARHGAP18 Antibody (Center) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (ARHGAP18 Antibody (Center) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-ARHGAP18 antibody
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.
Product Categories/Family for anti-ARHGAP18 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
74,977 Da
NCBI Official Full Name
ARHGAP18 protein
NCBI Official Synonym Full Names
Rho GTPase activating protein 18
NCBI Official Symbol
ARHGAP18
NCBI Official Synonym Symbols
MacGAP; FLJ25728; MGC126757; MGC138145; bA307O14.2
NCBI Protein Information
rho GTPase-activating protein 18; OTTHUMP00000017190; rho-type GTPase-activating protein 18
UniProt Protein Name
Rho GTPase-activating protein 18
UniProt Gene Name
ARHGAP18
UniProt Entry Name
RHG18_HUMAN

NCBI Description

ARHGAP18 belongs to a family of Rho (see MIM 165390) GTPase-activating proteins that modulate cell signaling (Potkin et al., 2009 [PubMed 19065146]).[supplied by OMIM]

Uniprot Description

ARHGAP18: a GTPase activator for the Rho-type GTPases, converting them to inactive GDP-bound states. Two alternatively-spliced isoforms have been described.

Protein type: GAPs, Rac/Rho; GAPs

Chromosomal Location of Human Ortholog: 6q22.33

Cellular Component: cytosol

Molecular Function: GTPase activator activity

Biological Process: regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of GTPase activity

Research Articles on ARHGAP18

Similar Products

Product Notes

The ARHGAP18 arhgap18 (Catalog #AAA623933) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARHGAP18, ID (Rho GTPase-activating Protein 18, Rho-type GTPase-activating Protein 18, MacGAP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARHGAP18 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500. Researchers should empirically determine the suitability of the ARHGAP18 arhgap18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARHGAP18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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