Rabbit Adrenergic Receptor, Alpha 2a Polyclonal Antibody | anti-ADRA2A antibody
Adrenergic Receptor, Alpha 2a (A2aAR)
Purified by immunoaffinity chromatography
Purified by immunoaffinity chromatography
Dilution: Immunohistochemistry: 1:100-1:500; Antiserum was used on perfusion fixed tissue. Perfusion: 1) Calcium-free Tyrode's solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS.
Slide mounted tissue sections were processed for indirect immunoflourescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4 degree C with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and cover-slipped. Staining was examined using fluorescence microscopy.
NCBI and Uniprot Product Information
NCBI Description
Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons; the alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes alpha2A subtype and it contains no introns in either its coding or untranslated sequences. [provided by RefSeq]
Uniprot Description
ADRA2A: Alpha-2 adrenergic receptors mediate the catecholamine- induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2A sub-subfamily.
Protein type: Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR
Chromosomal Location of Human Ortholog: 10q25.2
Cellular Component: basolateral plasma membrane; integral to plasma membrane; cytoplasm; plasma membrane; synapse; receptor complex
Molecular Function: protein binding; protein homodimerization activity; alpha-2C adrenergic receptor binding; protein heterodimerization activity; thioesterase binding; alpha2-adrenergic receptor activity; norepinephrine binding; alpha-1B adrenergic receptor binding; epinephrine binding; protein kinase binding; heterotrimeric G-protein binding
Biological Process: fear response; negative regulation of calcium ion-dependent exocytosis; positive regulation of potassium ion transport; thermoception; female pregnancy; signal transduction; glucose homeostasis; positive regulation of vasodilation; positive regulation of membrane protein ectodomain proteolysis; Rho protein signal transduction; intestinal absorption; negative regulation of insulin secretion; positive regulation of MAP kinase activity; negative regulation of cAMP biosynthetic process; positive regulation of epidermal growth factor receptor activity; negative regulation of lipid catabolic process; positive regulation of cell proliferation; DNA replication; positive regulation of cytokine production; platelet activation; negative regulation of calcium ion transport; activation of protein kinase B; negative regulation of adenylate cyclase activity; cellular response to hormone stimulus; G-protein coupled receptor protein signaling pathway; regulation of vasoconstriction; Ras protein signal transduction; activation of protein kinase activity; negative regulation of epinephrine secretion; energy reserve metabolic process; blood coagulation; actin cytoskeleton organization and biogenesis; cell motility; regulation of insulin secretion; acute inflammatory response; positive regulation of cell migration
Research Articles on ADRA2A
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Product Notes
The ADRA2A adra2a (Catalog #AAA622626) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Adrenergic Receptor, Alpha 2a (A2aAR) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Adrenergic Receptor, Alpha 2a can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunohistochemistry and Immunocytochemistry. Dilution: Immunohistochemistry: 1:100-1:500; Antiserum was used on perfusion fixed tissue. Perfusion: 1) Calcium-free Tyrode's solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide mounted tissue sections were processed for indirect immunoflourescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4 degree C with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and cover-slipped. Staining was examined using fluorescence microscopy. Researchers should empirically determine the suitability of the ADRA2A adra2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Adrenergic Receptor, Alpha 2a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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