Human Melanotransferrin ELISA Kit | MFI2 elisa kit

Human Melanotransferrin ELISA Kit

Cat. Num. AAA62206

• Gene Names: MFI2; MTF1; CD228; MAP97
• Reactivity: Human
• Synonyms: Melanotransferrin; Human Melanotransferrin ELISA Kit; MFI2 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human MFI2 and analogues was observed.
• Sequence Length: 738

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 62.5pg/ml-2000pg/ml.
• Sensitivity: 10pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated MFI2 concentrations in Human serum, plasma and other body fluids. Using Purified Human MFI2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MFI2 and MFI2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MFI2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for MFI2 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated MFI2 concentrations in Human serum, plasma and other body fluids. Using Purified Human MFI2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MFI2 and MFI2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MFI2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 134244281
• NCBI GeneID: 4241
• NCBI Accession #: NP_005920.2
• NCBI GenBank Nucleotide #: NM_005929.5
• UniProt Accession #: P08582; Q9BQE2
• Molecular Weight: 80,215 Da
• NCBI Official Full Name: melanotransferrin isoform 1
• NCBI Official Synonym Full Names: antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5
• NCBI Official Symbol: MFI2
• NCBI Official Synonym Symbols: MTF1; CD228; MAP97
• NCBI Protein Information: melanotransferrin; membrane-bound transferrin-like protein; melanoma-associated antigen p97, isoform 2
• UniProt Protein Name: Melanotransferrin
• Protein Family: Melanotransferrin
• UniProt Gene Name: MFI2
• UniProt Synonym Gene Names: MAP97
• UniProt Entry Name: TRFM_HUMAN

NCBI Description

The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

MFI2: Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 3q28-q29

Cellular Component: cell surface; anchored to plasma membrane; integral to plasma membrane

Molecular Function: protein binding; ferric iron binding; iron ion binding

Biological Process: cellular iron ion homeostasis; regulation of cell growth; regulation of cell proliferation