Rabbit anti-Mouse MAGP1 Polyclonal Antibody | anti-MFAP2 antibody

MAGP1

Cat. Num. AAA621978

• Gene Names: MFAP2; MAGP; MAGP1; MAGP-1
• Reactivity: Mouse
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
• Purity: Serum
• Synonyms: MAGP1; Polyclonal Antibody; Anti -MAGP1; anti-MFAP2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse MAGP-1. Limited cross-reactivity with human and bovine MAGP-1.
• Purity/Purification: Serum
• Form/Format: Supplied as a lyophilized powder. Reconstitute with 500ul sterile PBS.

• Applicable Applications for anti-MFAP2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: ELISA: 1:1000 -1:2000; Western Blot: 1:200 -1:500; Immunoprecipitation: 1:100 -1:200; Immunohistochemistry/Immunocytochemistry: 1:50 -1:100; Optimal dilutions to be determined by the researcher.; ; Note: For IHC and IC: In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6M guanidine HCl, 50mM dithiothreitol, 20mM Tris pH 8.0 for 15 minutes. After washing with 20mM Tris pH 8.0, the slides are treated with 100mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols.
• Immunogen: Amino-terminal half of mouse MAGP-1.
• Preparation and Storage: Lyophilized powder may be stored at -20°C. Stable for 12 months at after receipt -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-MFAP2 antibody

MAGP-1 and fibrillin-1, two protein components of extracellular microfibrils, were shown by immunoprecipitation studies to interact with the chondroitin sulfate proteoglycan decorin in the medium of cultured fetal bovine chondrocytes. Decorin interacted with each protein individually and with both proteins together to form a ternary complex. Expression of truncated fibrillin-1 proteins in Chinese hamster ovary cells localized proteoglycan binding to an amino-terminal region near the proline-rich domain. A spatially analogous fibrillin-2 truncated protein did not coprecipitate the same sulfated molecule, suggesting that chondroitin sulfate proteoglycan binding in this region is specific for fibrillin-1. An interaction between fibrillin and MAGP-1 was also observed under culture conditions that abrogated decorin secretion, suggesting that the two microfibrillar proteins can associate in the absence of the proteoglycan. Sulfation of matrix proteins is important for elastic fiber assembly because inhibition of sulfation was shown to prevent microfibrillar protein incorporation into the extracellular matrix of cultured cells.

Product Categories/Family for anti-MFAP2 antibody

Antibodies; Abs to Elastin

NCBI and Uniprot Product Information

• NCBI GI #: 207028935
• NCBI GeneID: 4237
• NCBI Accession #: NP_001128720.1
• NCBI GenBank Nucleotide #: NM_001135248.1
• UniProt Accession #: P55001; Q53X60; Q5JXY0
• NCBI Official Full Name: microfibrillar-associated protein 2 isoform b
• NCBI Official Synonym Full Names: microfibrillar-associated protein 2
• NCBI Official Symbol: MFAP2
• NCBI Official Synonym Symbols: MAGP; MAGP1; MAGP-1
• NCBI Protein Information: microfibrillar-associated protein 2; microfibril-associated glycoprotein 1
• UniProt Protein Name: Microfibrillar-associated protein 2
• Protein Family: Microfibrillar-associated protein
• UniProt Gene Name: MFAP2
• UniProt Synonym Gene Names: MAGP1; MFAP-2; MAGP; MAGP-1
• UniProt Entry Name: MFAP2_HUMAN

NCBI Description

Microfibrillar-associated protein 2 is a major antigen of elastin-associated microfibrils and a candidate for involvement in the etiology of inherited connective tissue diseases. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

MFAP2: Component of the elastin-associated microfibrils. Belongs to the MFAP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.1-p35

Cellular Component: extracellular region; microfibril

Molecular Function: fibronectin binding

Biological Process: extracellular matrix organization and biogenesis; platelet formation