Rabbit Vinculin Polyclonal Antibody | anti-VCL antibody

Vinculin, phosphorylated (Tyr822)

Cat. Num. AAA621491

• Gene Names: VCL; MVCL; CMD1W; CMH15
• Reactivity: Chicken, Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Vinculin; Polyclonal Antibody; phosphorylated (Tyr822); Anti -Vinculin; anti-VCL antibody

• Host: Rabbit
• Reactivity: Chicken, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes chicken vinculin when phosphorylated at Tyr822. Species sequence homology: human, mouse, and rat vinculin (100%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.

• Applicable Applications for anti-VCL antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic phosphopeptide derived from a region of human vinculin that contains tyrosine 822.
• Positive Control: Chick Embryo Fibroblasts (CEFs) transfected with activated Src
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-VCL antibody

Vinculin is an ubiquitously expressed cytoskeletal protein (~130kD) involved in cell adhesion and cell migration. The vinculin protein consists of a globular head domain connected to an elongated tail region by a proline-rich domain. The head region contains binding sites for two cytoskeletal proteins, a-actinin and talin, as well as a binding site for the tail region of vinculin itself. The linker region possesses binding sites for two adaptor proteins, vinexin and ponsin, that may connect growth factor receptors with integrin signaling pathways, and a binding site for an actin-bundling protein, VASP. The tail region contains binding sites for actin, the cytoskeletal protein, paxillin, and PI(4,5)P2. In the inactive state the head region of vinculin is bound to the tail region, resulting in inaccessibility of the other protein binding sites. Binding of PI(4,5)P2 releases the head-tail interaction allowing binding of other proteins to vinculin. These regulatory events play an important role in the formation, maintenance, and breakdown of focal adhesions that occur during cell adhesion and migration. In addition to these protein binding sites, the head and tail regions of vinculin have multiple potential phosphorylation sites. Data indicate the possible involvement of phosphorylation of tyrosine 822 of vinculin with the integrity/strength of integrinmediated focal adhesions.

Product Categories/Family for anti-VCL antibody

Antibodies; Abs to Membrane Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 246714
• NCBI GeneID: 7414
• UniProt Accession #: P18206; Q16450; Q5SWX2; Q7Z3B8; Q8IXU7; Q5JQ13
• Molecular Weight: 123,799 Da
• NCBI Official Full Name: vinculin
• NCBI Official Synonym Full Names: vinculin
• NCBI Official Symbol: VCL
• NCBI Official Synonym Symbols: MVCL; CMD1W; CMH15
• NCBI Protein Information: vinculin; metavinculin; OTTHUMP00000019861; OTTHUMP00000019862
• UniProt Protein Name: Vinculin
• Protein Family: Vinculin
• UniProt Gene Name: VCL
• UniProt Entry Name: VINC_HUMAN

NCBI Description

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]

Uniprot Description

Vinculin: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Exhibits self-association properties. Interacts with NRAP and SORBS1. Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Metavinculin is muscle-specific. Belongs to the vinculin/alpha-catenin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: costamere; focal adhesion; protein complex; extracellular region; intercellular junction; fascia adherens; cytosol; cell-matrix junction; actin cytoskeleton; cytoskeleton; cell-cell adherens junction; adherens junction; plasma membrane; vesicle

Molecular Function: protein binding; cadherin binding; beta-catenin binding; structural molecule activity; actin binding; alpha-catenin binding

Biological Process: lamellipodium biogenesis; platelet activation; platelet degranulation; apical junction assembly; muscle contraction; axon extension; cell-matrix adhesion; morphogenesis of an epithelium; cell adhesion; blood coagulation; cell motility; negative regulation of cell migration

Disease: Cardiomyopathy, Dilated, 1w