Western Blot (WB)
(WB: Recombinant Xenopus Histone H2B (lane 1), chicken core histones (lane 2) and acid extract from either sodium butyrate (lane 3) or colcemid treated (lane 4) HeLa cells were probed with anti-Histone H2B (1:5,000 dilution).)
Rabbit Histone H2B, aa118-126 Polyclonal Antibody | anti-HTB2 antibody
Histone H2B, aa118-126
Gene Names
HTB2; H2B; histone B2; HISTONE H2B; HTB2; MRN17.11; MRN17_11
Reactivity
Chicken, Human, Xenopus
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Histone H2B; aa118-126; Polyclonal Antibody; Anti -Histone H2B; anti-HTB2 antibody
Host
Rabbit
Reactivity
Chicken, Human, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes and is specific for Histone H2B, Mr ~15kD. An additional band at 23kD is
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in 200ul of 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide
Applicable Applications for anti-HTB2 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blotting.
Dilution: Western Blot : 1:1,000-1:10,000 detected Histone H2B in acid extracts from either sodium butyrate or colcemid treated HeLa cells and 13-114 respectively), chicken core histone and recombinant Xenopus Histone H2B
Dilution: Western Blot : 1:1,000-1:10,000 detected Histone H2B in acid extracts from either sodium butyrate or colcemid treated HeLa cells and 13-114 respectively), chicken core histone and recombinant Xenopus Histone H2B
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids 118-126 (CGAVTKYTSSK) of human Histone H2B, with an Nterminal CG added for conjugation purposes.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(WB: Recombinant Xenopus Histone H2B (lane 1), chicken core histones (lane 2) and acid extract from either sodium butyrate (lane 3) or colcemid treated (lane 4) HeLa cells were probed with anti-Histone H2B (1:5,000 dilution).)
Western Blot (WB)
(WB: Recombinant Xenopus Histone H2B (lane 1), chicken core histones (lane 2) and acid extract from either sodium butyrate (lane 3) or colcemid treated (lane 4) HeLa cells were probed with anti-Histone H2B (1:5,000 dilution).)
Product Categories/Family for anti-HTB2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
histone H2B
NCBI Official Symbol
HTB2
NCBI Official Synonym Symbols
H2B; histone B2; HISTONE H2B; HTB2; MRN17.11; MRN17_11
NCBI Protein Information
histone H2B
UniProt Protein Name
Histone H2B.10
UniProt Entry Name
H2B10_ARATH
Uniprot Description
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Research Articles on HTB2
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Product Notes
The HTB2 (Catalog #AAA621287) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2B, aa118-126 reacts with Chicken, Human, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2B, aa118-126 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blotting. Dilution: Western Blot : 1:1,000-1:10,000 detected Histone H2B in acid extracts from either sodium butyrate or colcemid treated HeLa cells and 13-114 respectively), chicken core histone and recombinant Xenopus Histone H2B. Researchers should empirically determine the suitability of the HTB2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2B, aa118-126, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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