Rabbit anti-Human Telomeric Repeat Binding Factor 1 Polyclonal Antibody | anti-TERF1 antibody

Telomeric Repeat Binding Factor 1 (TRF1, RAP1)

Cat. Num. AAA620587

• Gene Names: TERF1; TRF; PIN2; TRF1; TRBF1; t-TRF1; FLJ41416; hTRF1-AS
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Telomeric Repeat Binding Factor 1; Polyclonal Antibody; Telomeric Repeat Binding Factor 1 (TRF1; RAP1); Anti -Telomeric Repeat Binding Factor 1 (TRF1; anti-TERF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Telomeric Repeat Binding Factor 1 (TRF1, RAP1). No significant sequence homology with TRF2.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.

• Applicable Applications for anti-TERF1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1-10ug/ml using ECL.; ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml.
• Immunogen: 20aa peptide sequence near the C-terminus of human TRF1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-TERF1 antibody

TRF1 is a negative regulator of telomere length maintenance (1). TRF1 has a number of biochemical similarities to Rap1p, a distantly related DNA-binding protein that functions at telomeres in yeast (1,2). Like Rap1p, TRF1 requires two Myb motifs for DNA binding. Recently two proteins, tankyrase and Tin2, which bind to mammalian TRF1, have been identified. Tankyrase is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP) (3). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats (3,4). Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP)-ribosylation. Since ADP-ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP)-ribosylation. Tin2 localizes to telomeres and is essential for proper regulation of telomere length. TIN2, like TRF1, is widely and constitutively expressed, suggesting that these proteins act together to counterbalance telomere elongation by telomerase. Recently, it has been shown that TRF1 expression is significantly elevated in patients with acute lymphoblastic leukemia (ALL) compared to those in acute myeloid leukemia (AML) (5). In this study, TRF1 expression tended to be higher in patients without telomere shortening and in those with hTERT expression suggesting that TRF1 may act to monitor telomere length under the condition of up-regulated telomerase activity in some neoplastic cells.

Product Categories/Family for anti-TERF1 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 189409142
• NCBI GeneID: 7013
• NCBI Accession #: NP_059523.2
• NCBI GenBank Nucleotide #: NM_017489.2
• UniProt Accession #: P54274; Q15553; Q8NHT6; Q93029; A7XP29; Q5QGN5
• Molecular Weight: 50,246 Da
• NCBI Official Full Name: telomeric repeat-binding factor 1 isoform 1
• NCBI Official Synonym Full Names: telomeric repeat binding factor (NIMA-interacting) 1
• NCBI Official Symbol: TERF1
• NCBI Official Synonym Symbols: TRF; PIN2; TRF1; TRBF1; t-TRF1; FLJ41416; hTRF1-AS
• NCBI Protein Information: telomeric repeat-binding factor 1; OTTHUMP00000226889; OTTHUMP00000226890; NIMA-interacting protein 2; telomeric protein Pin2/TRF1; TTAGGG repeat-binding factor 1
• UniProt Protein Name: Telomeric repeat-binding factor 1
• Protein Family: Telomeric repeat-binding factor
• UniProt Gene Name: TERF1
• UniProt Synonym Gene Names: PIN2; TRBF1; TRF; TRF1
• UniProt Entry Name: TERF1_HUMAN

NCBI Description

This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products. [provided by RefSeq]

Uniprot Description

TRF1: a telomeric repeat binding factor. Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Involved in the regulation of the mitotic spindle. Expression is tightly regulated during the cell cycle; levels are low in G1 and S phase and increase during G2 phase and mitosis. Two splice variant isoforms have been described.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 8q21.11

Cellular Component: nucleoplasm; chromosome, telomeric region; nuclear telomere cap complex; nuclear chromosome, telomeric region; cytoplasm; nucleolus; spindle; nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; microtubule binding; telomeric DNA binding; double-stranded telomeric DNA binding; chromatin binding; DNA bending activity

Biological Process: response to drug; mitosis; positive regulation of mitosis; positive regulation of apoptosis; negative regulation of telomere maintenance via semi-conservative replication; positive regulation of microtubule polymerization; telomere maintenance via telomerase; positive regulation of mitotic cell cycle; negative regulation of telomerase activity; negative regulation of DNA replication; cell division; telomeric loop formation; mitotic cell cycle spindle assembly checkpoint; age-dependent telomere shortening; G2/M transition of mitotic cell cycle; negative regulation of telomere maintenance via telomerase; telomere maintenance; protein homooligomerization