anti-Mouse ABCG4 Polyclonal Antibody | anti-ABCG4 antibody

Anti - ABCG4 (ATP-binding Cassette (ABC) Transporter 4)

Cat. Num. AAA619548

• Gene Names: ABCG4; WHITE2
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Purified by immunoaffinity chromatography.
• Synonyms: ABCG4; Polyclonal Antibody; Anti - ABCG4 (ATP-binding Cassette (ABC) Transporter 4); ABCG4 (ATP-binding Cassette (ABC) Transporter 4); anti-ABCG4 antibody

• Host: Host: Rabbit; Source: Mouse
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: The control peptide is 100% conserved in mouse, 93% in rat and 80% in human ABCG4.
• Purity/Purification: Purified by immunoaffinity chromatography.
• Form/Format: Affinity Purified; Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
• Concentration: ~1mg/ml (varies by lot)
• Sequence Length: 646

• Applicable Applications for anti-ABCG4 antibody: ELISA, Western Blot
• Application Notes: Suitable for use in Western Blot and ELISA. Other applications not tested.; Dilution: Western Blot: (1-10ug/ml using ECL technique).; ELISA: Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies (0.5-1ug/ml for affinity pure).; Optimal dilution determined by the researcher.
• Immunogen: 15-aa peptide from Mouse ABCG4 ~C-terminus, Extracellular
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-ABCG4 antibody

The ATP binding cassette (ABC) superfamily of membrane transporters is one of the largest protein classes known, and counts for numerous proteins involved in trafficking of biological molecules across membranes, host-defense mechanism to xenobiotics. The first known members were P-glycoprotein (P-gp) and multidrug resistant protein (MRP), cause multidrug resistance when transfected into drug-sensitive cells. In addition, increasing numbers of ABC proteins have recently been identified. The human ABCG1 (ABC, subfamily G, member 1) gene encodes a member of ABC superfamily that mediates the ATP-dependent translocation of variety of amphiphilic and lipophilic molecules. ABCG2 has been identified as a candidate protein responsible for cancer multidrug resistance, the overexpression of ABCG2 was found in several drug-selective cell lines. Search made of EST databases with BLAST program led to identification of several mouse and rat sequences that had high homology to ABCG2 but that appeared to encode a unique gene. ABCG3 is the most closely related to ABCG2 with 54% amino acid identity overall. The gene, ABCG4, produces several transcripts that differ at the 5' end and encode proteins of various lengths, the ABCG4 protein is closely related to the Drosophila's white and human ABCG1 genes, and belongs to the ABCG subfamily which are involved in cholesterol transport. ABCG5 and ABCG8 are members of the G subfamily of ABC transporters, which are predicted to contain a single magnesium-dependent ATP catalytic domain N-terminal to six transmembrane segments, mutations in either of them cause an identical phenotype which is consistent with these two gene products functioning as heterodimer. ABCG6 and ABCG7 exist in Dictyostelium species of eukaryotes.

Product Categories/Family for anti-ABCG4 antibody

Abs to Transporters

References

• Annilo T et al (2001) Lab. Of Genomic Diversity, NCI-Frederick, MD 21702, USA; Engel T et al (Unpublished) Inst. Fur Arterioskleroseforschung, Germany; L. Austin Doyle (1998) PNAS Vol. 95, 15665-15670.

NCBI and Uniprot Product Information

• NCBI GI #: 215820660
• NCBI GeneID: 64137
• NCBI Accession #: NP_001135977.1
• NCBI GenBank Nucleotide #: NM_001142505.1
• UniProt Accession #: Q9H172; A8K1B5; Q8WWH0; Q8WWH1; Q8WWH2; Q9NT30
• Molecular Weight: 71,896 Da
• NCBI Official Full Name: ATP-binding cassette sub-family G member 4
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family G (WHITE), member 4
• NCBI Official Symbol: ABCG4
• NCBI Official Synonym Symbols: WHITE2
• NCBI Protein Information: ATP-binding cassette sub-family G member 4; OTTHUMP00000232083; OTTHUMP00000232085; OTTHUMP00000232086; putative ABC transporter; ATP-binding cassette, subfamily G, member 4
• UniProt Protein Name: ATP-binding cassette sub-family G member 4
• Protein Family: ABC transporter G family
• UniProt Gene Name: ABCG4
• UniProt Synonym Gene Names: WHITE2
• UniProt Entry Name: ABCG4_HUMAN