Goat anti-Human AKAP3 Polyclonal Antibody | anti-AKAP3 antibody

AKAP3 (SOB1, FSP95, PRKA3, AKAP110, A Kinase (PRKA) Anchor Protein 3, Fibrousheathin 1, Sperm Oocyte Binding Protein 1)

Cat. Num. AAA619013

• Gene Names: AKAP3; CT82; SOB1; FSP95; PRKA3; AKAP110
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: AKAP3; Polyclonal Antibody; AKAP3 (SOB1; FSP95; PRKA3; AKAP110; A Kinase (PRKA) Anchor Protein 3; Fibrousheathin 1; Sperm Oocyte Binding Protein 1); Anti -AKAP3 (SOB1; anti-AKAP3 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human AKAP3 at ~90kD in Human Testis lysates (calculated MW of 95kD according to NP_006413).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-AKAP3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:32000; Western Blot: 1-3ug/ml
• Immunogen: Synthetic peptide corresponding to human AKAP3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of AKAP3 in Human Testis lysate (35μg protein in RIPA buffer) using AKAP3 antibody (1μg/ml).)

Related Product Information for anti-AKAP3 antibody

A-kinase anchor proteins (AKAPs) direct the activity of protein kinase A (PKA) by tethering the enzyme near its physiologic substrates. May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction in sperm.

Product Categories/Family for anti-AKAP3 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 21493041
• NCBI GeneID: 10566
• NCBI Accession #: NP_006413.2
• NCBI GenBank Nucleotide #: NM_006422.2
• UniProt Accession #: O75969; O75945; Q86X01; Q9UM61
• Molecular Weight: 94,751 Da
• NCBI Official Full Name: A-kinase anchor protein 3
• NCBI Official Synonym Full Names: A kinase (PRKA) anchor protein 3
• NCBI Official Symbol: AKAP3
• NCBI Official Synonym Symbols: CT82; SOB1; FSP95; PRKA3; AKAP110
• NCBI Protein Information: A-kinase anchor protein 3; AKAP-3; AKAP 110; fibrousheathin 1; fibrousheathin I; fibrousheathin-1; OTTHUMP00000238368; cancer/testis antigen 82; fibrous sheath protein, 95kDa; sperm oocyte-binding protein 1; A-kinase anchor protein 110 kDa; A-kinase anchor protein, 110kDa; Fibrous Sheath Protein of 95 kDa; protein kinase A anchoring protein 3; protein kinase A-anchoring protein 3
• UniProt Protein Name: A-kinase anchor protein 3
• Protein Family: A-kinase anchor protein
• UniProt Gene Name: AKAP3
• UniProt Synonym Gene Names: AKAP110; SOB1
• UniProt Entry Name: AKAP3_HUMAN

NCBI Description

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family, and is expressed in testis only. The encoded protein contains an RII-binding domain, and is predicted to participate in protein-protein interactions with the R-subunit of the PKA. This protein is localized to the ribs of the fibrous sheath in the principal piece of the sperm tail. It may function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction. [provided by RefSeq]

Uniprot Description

AKAP3: May function as a regulator of both motility- and head- associated functions such as capacitation and the acrosome reaction. Belongs to the AKAP110 family.

Protein type: Adaptor/scaffold; Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: cytoplasm; acrosome; nucleus

Molecular Function: protein binding; protein kinase A binding

Biological Process: protein localization; single fertilization; acrosome reaction; transmembrane receptor protein serine/threonine kinase signaling pathway; cell motility