Rabbit HEY 2 Polyclonal Antibody | anti-SUV420H1 antibody

HEY 2 (Helix-loop-helix Transcription Factor, Hairy/enhancer-of-split related with YRPW motif 2, bHLHb32, BHLHB32, Cardiovascular helix-loop-helix factor 1, CHF1, Class B basic helix-loop-helix protein 32, GRIDLOCK, GRL, Hairy and enhancer of split-relate

Cat. Num. AAA618756

• Gene Names: SUV420H1; CGI85; KMT5B; CGI-85
• Reactivity: Human, Rodent
• Applications: Immunohistochemistry
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: HEY 2; Polyclonal Antibody; HEY 2 (Helix-loop-helix Transcription Factor; Hairy/enhancer-of-split related with YRPW motif 2; bHLHb32; BHLHB32; Cardiovascular helix-loop-helix factor 1; CHF1; Class B basic helix-loop-helix protein 32; GRIDLOCK; GRL; Hairy and enhancer of split-relate; Anti -HEY 2 (Helix-loop-helix Transcription Factor; anti-SUV420H1 antibody

• Host: Rabbit
• Reactivity: Human, Rodent
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Hey 2/HRT2. Species Crossreactivity: rodent.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added.

• Applicable Applications for anti-SUV420H1 antibody: Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry.; Dilution: Immunohistochemistry: 1:200-1:1000
• Immunogen: Synthetic peptide corresponding to aa314-330 of human Hey 2/HRT2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-SUV420H1 antibody

The Hey family of bHLH transcription factors are direct targets of Notch signaling. HEY2 (gridlock) is an important determinant of mammalian heart development, loss of which in mouse leads to cardiac defects with high postnatal lethality. Mouse Hey1 knockouts have no apparent phenotypic defect but the combined loss of Hey1 and Hey2 results in embryonic death after embryonic day 9.5 (E9.5) with a global lack of vascular remodeling and massive hemorrhage. Similar defects are observed in Jagged1 and Notch1 knockout mice.

Product Categories/Family for anti-SUV420H1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 50659084
• NCBI GeneID: 51111
• NCBI Accession #: NP_057112.3
• NCBI GenBank Nucleotide #: NM_016028.4
• UniProt Accession #: Q4FZB7; Q3SX56; Q4V775; Q6P150; Q96E44; Q9BUL0; Q9H022; Q9H2K3; Q9NXV3; Q9Y393; B7WNX7
• Molecular Weight: 99,187 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase SUV420H1 isoform 2
• NCBI Official Synonym Full Names: suppressor of variegation 4-20 homolog 1 (Drosophila)
• NCBI Official Symbol: SUV420H1
• NCBI Official Synonym Symbols: CGI85; KMT5B; CGI-85
• NCBI Protein Information: histone-lysine N-methyltransferase SUV420H1; suv4-20h1; C630029K18Rik; su(var)4-20 homolog 1; lysine N-methyltransferase 5B
• UniProt Protein Name: Histone-lysine N-methyltransferase SUV420H1
• UniProt Gene Name: SUV420H1
• UniProt Synonym Gene Names: KMT5B; Su(var)4-20 homolog 1; Suv4-20h1
• UniProt Entry Name: SV421_HUMAN

NCBI Description

This gene encodes a protein that contains a SET domain. SET domains appear to be protein-protein interaction domains that mediate interactions with a family of proteins that display similarity with dual-specificity phosphatases (dsPTPases). The function of this gene has not been determined. Two alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SUV420H1: a protein methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Strongly down-regulated in breast cancer cells. Belongs to the histone-lysine methyltransferase family. Suvar4-20 subfamily. Three alternatively-spliced isoforms have been described.

Protein type: Methyltransferase; Methyltransferase, protein lysine; EC 2.1.1.43; Amino Acid Metabolism - lysine degradation

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: condensed nuclear chromosome, pericentric region

Molecular Function: histone lysine N-methyltransferase activity (H4-K20 specific); histone-lysine N-methyltransferase activity

Biological Process: muscle development; transcription, DNA-dependent; regulation of transcription, DNA-dependent