Sheep Protein Phosphatase 2C delta, Serine, Threonine, Polyclonal Antibody | anti-PPM1D antibody

Protein Phosphatase 2C delta, Serine, Threonine, (PP2Cd Ser/Thr, ILKAP, integrin-linked kinase-associated serine/threonine phosphatase)

Cat. Num. AAA618473

• Gene Names: PPM1D; WIP1; PP2C-DELTA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Affinity purified on a column derivatized with the recombinant protein and further purified by
• Synonyms: Protein Phosphatase 2C delta; Serine; Threonine; ; Polyclonal Antibody; (PP2Cd Ser/Thr; ILKAP; integrin-linked kinase-associated serine/threonine phosphatase); Anti -Protein Phosphatase 2C delta; anti-PPM1D antibody

• Host: Sheep
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human, mouse and rat ILKAP. detects endogenous human ILKAP at 47 kDa using Western blot. This is higher than the predicted molecular weight of 43 kDa but is consistent with the electrophoretic migration of rhILKAP. By direct ELISA, the antibody is selective for ILKAP over PP2C alpha, beta and gamma by 25-, 16-, and 92-fold, respectively.
• Purity/Purification: Affinity Purified; Affinity purified on a column derivatized with the recombinant protein and further purified by
• Form/Format: Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with PBS containing 0.02% NaN3.

• Applicable Applications for anti-PPM1D antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western blot.; Dilution: Western Blot: 1ug/ml
• Immunogen: E. coli-derived recombinant human rhILKAP (aa 1- 392).
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-PPM1D antibody

Integrin Linked Kinase 1 Associated Phosphatase (ILKAP), also called PP2Cdelta, is a

Product Categories/Family for anti-PPM1D antibody

Antibodies; Abs to Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 119571794
• NCBI GeneID: 8493
• UniProt Accession #: O15297; Q53XP4
• Molecular Weight: 66,675 Da
• NCBI Official Full Name: protein phosphatase 1D magnesium-dependent, delta isoform, isoform CRA_b
• NCBI Official Synonym Full Names: protein phosphatase, Mg2+/Mn2+ dependent, 1D
• NCBI Official Symbol: PPM1D
• NCBI Official Synonym Symbols: WIP1; PP2C-DELTA
• NCBI Protein Information: protein phosphatase 1D; protein phosphatase Wip1; wild-type p53-induced phosphatase 1; protein phosphatase 2C delta isoform; protein phosphatase 1D magnesium-dependent, delta isoform
• UniProt Protein Name: Protein phosphatase 1D
• Protein Family: Protein phosphatase
• UniProt Gene Name: PPM1D
• UniProt Synonym Gene Names: WIP1; PP2C-delta
• UniProt Entry Name: PPM1D_HUMAN

NCBI Description

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development. [provided by RefSeq, Jul 2008]

Uniprot Description

PPM1D: a magnesium-dependent Ser/Thr phosphatase. A p53-induced phosphatase that interacts with the nuclear isoform of uracil DNA glycosylase, UNG2, and suppresses base excision repair. Might contribute to growth inhibitory pathways activated in response to DNA damage in a p53-dependent manner.

Protein type: Motility/polarity/chemotaxis; Cell cycle regulation; Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 17q23.2

Cellular Component: nucleus

Molecular Function: protein binding; metal ion binding; protein serine/threonine phosphatase activity

Biological Process: negative regulation of cell proliferation; response to radiation; response to bacterium; G2/M transition of mitotic cell cycle; protein amino acid dephosphorylation

Disease: Breast Cancer