anti-Rat NHE3 Polyclonal Antibody | anti-NHE3 antibody
Anti - NHE3 (Na, H Exchanger 3)
Gene Names
Nhe3; CG11328; DmelCG11328; DmNHE3; nhe3; NHE3
Reactivity
Rat
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
NHE3; Polyclonal Antibody; Anti - NHE3 (Na; H Exchanger 3); NHE3 (Na; anti-NHE3 antibody
Host
Host: Chicken
Source: Rat
Source: Rat
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgY
Specificity
Recognizes NHE3. No significant sequence homology exist with other NHE isoforms (NHE1, 2, 4 and 5). Species Sequence Homology
Purity/Purification
Serum
Form/Format
Serum
Supplied as a liquid, neat serum, 40% glycerol.
Supplied as a liquid, neat serum, 40% glycerol.
Sequence Length
696
Applicable Applications for anti-NHE3 antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA, Western Blot. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 using ECL. A band of 95-110kD has been detected in brush border membrane (2).
ELISA: 1:100,000 Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Dilution: Western Blot: 1:1000-1:5000 using ECL. A band of 95-110kD has been detected in brush border membrane (2).
ELISA: 1:100,000 Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Immunogen
A synthetic peptide consisting of 22aa within the cytoplasmic, c-terminal domain of the rat NHE3 (1) (KLH coupled).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-NHE3 antibody
Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. Seven NHE isoforms (NHE1-7) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The COOH-terminals of NHE1, NHE2 and NHE3 are intracellular.
Product Categories/Family for anti-NHE3 antibody
References
• 1. Brant, S.R., et al. (1995) Am. J. Physiol. 269, C198-C206.
• 2. Orlowsji, J., et al. (1992) J. Biol. Chem. 267, 9331.
• 3. Chris Yun, C.H., et al. (1995) Am. J. Physiol. 269, G1-G11 (Review).
• 4. Josette, N. and Pouyssegur, J. (1995) Am. J. Physiol. 268, C283-C296 (review).
• 5. Yoshioka, et al. (1997) J. Biol. Chem. 122, 641.
• 2. Orlowsji, J., et al. (1992) J. Biol. Chem. 267, 9331.
• 3. Chris Yun, C.H., et al. (1995) Am. J. Physiol. 269, G1-G11 (Review).
• 4. Josette, N. and Pouyssegur, J. (1995) Am. J. Physiol. 268, C283-C296 (review).
• 5. Yoshioka, et al. (1997) J. Biol. Chem. 122, 641.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Na[+]/H[+] hydrogen exchanger 3, isoform H
NCBI Official Synonym Full Names
Na[+]/H[+] hydrogen exchanger 3
NCBI Official Symbol
Nhe3
NCBI Official Synonym Symbols
CG11328; DmelCG11328; DmNHE3; nhe3; NHE3
NCBI Protein Information
CG11328 gene product from transcript CG11328-RH; Na[+]/H[+] hydrogen exchanger 3, isoform H; CG11328-PH; Nhe3-PH; Na[+]/H[+] hydrogen exchanger 3, isoform G; CG11328-PG; Nhe3-PG; Na[+]/H[+] hydrogen exchanger 3, isoform E; CG11328-PE; Nhe3-PE; Na[+]/H[+] hydrogen exchanger 3, isoform D; CG11328-PD; Nhe3-PD
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Product Notes
The NHE3 (Catalog #AAA618320) is an Antibody produced from Host: Chicken Source: Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti - NHE3 (Na, H Exchanger 3) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NHE3 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA, Western Blot. Other applications not tested. Dilution: Western Blot: 1:1000-1:5000 using ECL. A band of 95-110kD has been detected in brush border membrane (2). ELISA: 1:100,000 Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the NHE3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NHE3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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