Mouse CD59 Monoclonal Antibody | anti-CD59 antibody

CD59 (CD59 Molecule, Complement Regulatory Protein, 16.3A5, 1F5, EJ16, EJ30, EL32, FLJ38134, FLJ92039, G344, HRF-20, HRF20, MAC-IP, MACIF, MEM43, MGC2354, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18-20) (HRP)

Cat. Num. AAA6182986

• Gene Names: CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
• Applications: Western Blot
• Purity: Purified
• Synonyms: CD59; Monoclonal Antibody; CD59 (CD59 Molecule; Complement Regulatory Protein; 16.3A5; 1F5; EJ16; EJ30; EL32; FLJ38134; FLJ92039; G344; HRF-20; HRF20; MAC-IP; MACIF; MEM43; MGC2354; MIC11; MIN1; MIN2; MIN3; MIRL; MSK21; p18-20) (HRP); CD59 Molecule; p18-20; anti-CD59 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 3G6
• Specificity: Recognizes CD59.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-CD59 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: CD59 (NP_000602.1, 1aa-128aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of CD59 expression in transfected 293T cell line by CD59 monoclonal antibody (M02), clone 3G6.Lane 1: CD59 transfected lysate (Predicted MW: 41.2 KDa).Lane 2: Non-transfected lysate.)

Western Blot (WB)

(CD59 monoclonal antibody (M02), clone 3G6. Western Blot analysis of CD59 expression in HeLa.)

Testing Data

(Detection limit for recombinant GST tagged CD59 is 1 ng/ml as a capture antibody.)

Product Categories/Family for anti-CD59 antibody

Antibodies; CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 10835165
• NCBI GeneID: 966
• NCBI Accession #: NP_000602.1
• NCBI GenBank Nucleotide #: NM_000611.5
• UniProt Accession #: P13987
• Molecular Weight: 14,177 Da
• NCBI Official Full Name: CD59 glycoprotein preproprotein
• NCBI Official Synonym Full Names: CD59 molecule, complement regulatory protein
• NCBI Official Symbol: CD59
• NCBI Official Synonym Symbols: 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
• NCBI Protein Information: CD59 glycoprotein; 1F5 antigen; 20 kDa homologous restriction factor; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344); Ly-6-like protein; MEM43 antigen; T cell-activating protein; human leukocyte antigen
• UniProt Protein Name: CD59 glycoprotein
• Protein Family: CD59 glycoprotein
• UniProt Gene Name: CD59
• UniProt Synonym Gene Names: MIC11; MIN1; MIN2; MIN3; MSK21; HRF-20; HRF20; MAC-IP; MACIF; MIRL
• UniProt Entry Name: CD59_HUMAN

NCBI Description

This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CD59: Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: anchored to external side of plasma membrane; compact myelin; extracellular space; cell surface; focal adhesion; membrane; extracellular region; plasma membrane; sarcolemma; vesicle

Molecular Function: complement binding; protein binding

Biological Process: cell activation; cell surface receptor linked signal transduction; regulation of complement activation; innate immune response; negative regulation of activation of membrane attack complex; positive regulation of T cell proliferation; blood coagulation; negative regulation of apoptosis

Disease: Hemolytic Anemia, Cd59-mediated, With Or Without Immune-mediated Polyneuropathy