Rabbit ZO-3, Mid Polyclonal Antibody | anti-TJP3 antibody

ZO-3, Mid (Tight Junction-associated Polypeptide, Zonula Occludens 3)

Cat. Num. AAA618203

• Gene Names: TJP3; ZO3; ZO-3; MGC119546
• Reactivity: Canine, Human
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ZO-3; Mid; Polyclonal Antibody; Mid (Tight Junction-associated Polypeptide; Zonula Occludens 3); Anti -ZO-3; anti-TJP3 antibody

• Host: Rabbit
• Reactivity: Canine, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ZO-3 protein with a molecular weight of ~130kD. Crossreactivity with other Zonula Occluden family members has not been observed. Reactivity is con rmed with human T84, HeLa, and canine MDCK cell lysates.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
• Concentration: 0.25mg/ml (varies by lot)

• Applicable Applications for anti-TJP3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation.; Dilution: Immunofluorescence: 0.5-2ug/ml; ELISA: 0.1-1ug/ml; Western Blot: 1-3ug/ml; Immunoprecipitation: 2-3ug; Optimal dilutions to be determined by the researcher.
• Immunogen: Synthetic peptide derived from the mid region of the human ZO-3 protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-TJP3 antibody

ZO-3, a ~130kD protein, contains three PSD-95/SAP90, discs-large, ZO-1 (PDZ) domains, a SH3 domain, and a guanylate kinase-liked domain. Like ZO-1 and ZO-2, ZO-3 contains a C-terminal acidic domain and a basic region (between the first and second PDZ domains), making it belongs to the MAGUK protein family1. By Western blot analysis, ZO-3 is identified as a ~130kDa protein that directly interacts with tight junction proteins ZO-1 and occuldin, but not with ZO-2. However, in one study, ZO-2, ZO-3 and Occludin colocalized with actin aggregates at cell border in MDCK cells2. ZO-3 is localized to sites of cell-cell interaction, identical to that of ZO-1 in tight junctions. By immunoprecipitation analysis, ZO-3 co-immunoprecipitates with ZO-1. Using MDCK cells, ZO-3 protein colocalized with ZO-1 by immunofluorescence. In vitro binding assays, the N-terminal of ZO-3 showed preferably binding of ZO-1 and actin filaments3. Moreover, the binding of ZO-1/ZO-2/ZO-3 was also found to interact with certain Claudin proteins.

Product Categories/Family for anti-TJP3 antibody

Antibodies; Abs to Connexin; Junction Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 3851202
• NCBI GeneID: 27134
• UniProt Accession #: O95049; Q32N01; A6NFP3; Q2VPE5; Q96KB4
• Molecular Weight: 102,800 Da
• NCBI Official Full Name: ZO-3
• NCBI Official Synonym Full Names: tight junction protein 3 (zona occludens 3)
• NCBI Official Symbol: TJP3
• NCBI Official Synonym Symbols: ZO3; ZO-3; MGC119546
• NCBI Protein Information: tight junction protein ZO-3; zona occludens 3; zona occludens protein 3; zonula occludens protein 3
• UniProt Protein Name: Tight junction protein ZO-3
• Protein Family: Tight junction protein
• UniProt Gene Name: TJP3
• UniProt Synonym Gene Names: ZO3
• UniProt Entry Name: ZO3_HUMAN

NCBI Description

TJP3 is a member of the family of membrane-associated guanylate kinase-like proteins (MAGUK) that associate with intracellular junctions (Itoh et al., 1999 [PubMed 10601346]).[supplied by OMIM]

Uniprot Description

ZO3: Interacts with occludin, claudins and ZO-1. Interacts with INADL. Interacts with UBN1. Interacts with FASLG. Belongs to the MAGUK family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; tight junction; apical plasma membrane; nucleus; cell junction

Molecular Function: protein binding