Rabbit anti-Human Hepcidin, 25aa Polyclonal Antibody
Hepcidin, 25aa (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide)
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Hepcidin; 25aa; Polyclonal Antibody; 25aa (Hepc; LEAP; Hepatic Bactericidal Protein; Liver Expressed Antimicrobial Peptide); Anti -Hepcidin; anti-Hepcidin; 25aa antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes all 3 processed forms of human HEPC. The human sequence is conserved in all 3 human HEPC peptides (20-, 22- and 25-aa C-terminal fragments). May react with the unprocessed larger 60aa pro-HEPC if present in the samples or tissues.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Hepcidin, 25aa antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Western Blot.
Dilution: Western Blot: 1-10ug/ml (ECL).
ELISA: 1:100,000. Using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Dilution: Western Blot: 1-10ug/ml (ECL).
ELISA: 1:100,000. Using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Immunogen
Synthetic peptide corresponding to13aa within the C-terminus of mature 25aa human HEPC, conjugated to KLH. Species Sequence Homology: mouse: 69%; rat: 61%.
Recommended Control Peptide
(Please Inquire) Hepcidin, 25aa, Human, Control Peptide (Hepc, LEAP, Heaptic Bactericidal Protein, Liver Expressed Antimicrobial Peptide)
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Product Categories/Family for anti-Hepcidin, 25aa antibody
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Product Notes
The Hepcidin, 25aa (Catalog #AAA617216) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Hepcidin, 25aa (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Hepcidin, 25aa can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Western Blot. Dilution: Western Blot: 1-10ug/ml (ECL). ELISA: 1:100,000. Using 50-100ng control peptide/well. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Hepcidin, 25aa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hepcidin, 25aa, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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