Western Blot (WB)
(Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using MBS616639.)
Rabbit Germinal Center Kinase Polyclonal Antibody
Germinal Center Kinase (GC Kinase, GCK, B Lymphocyte Serine/threonine-protein Kinase, BL44, Mitogen-activated Protein Kinase Kinase Kinase Kinase 2, MAP4K2, MAPK/ERK Kinase Kinase Kinase 2, MEK Kinase Kinase 2, MEKKK 2, Rab8-interacting Protein, RAB8IP)
Reactivity
Human, Monkey, Mouse, Rat
Applications
ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence
Purity
Affinity PurifiedPurified by protein A and peptide affinity chromatography.
Synonyms
Germinal Center Kinase; Polyclonal Antibody; Germinal Center Kinase (GC Kinase; GCK; B Lymphocyte Serine/threonine-protein Kinase; BL44; Mitogen-activated Protein Kinase Kinase Kinase Kinase 2; MAP4K2; MAPK/ERK Kinase Kinase Kinase 2; MEK Kinase Kinase 2; MEKKK 2; Rab8-interacting Protein; RAB8IP); Anti -Germinal Center Kinase (GC Kinase; anti-Germinal Center Kinase antibody
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes endogenous levels of total human GCK protein at 91kD. Does not crossreact with HPK or other members of the GCK family. Species Crossreactivity: mouse, rat, monkey.
Purity/Purification
Affinity Purified
Purified by protein A and peptide affinity chromatography.
Purified by protein A and peptide affinity chromatography.
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Applicable Applications for anti-Germinal Center Kinase antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry.
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:50
Immunocytochemistry (IF): 1:100
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:50
Immunocytochemistry (IF): 1:100
Immunogen
Synthetic peptide (KLH coupled) corresponding to residues surrounding Ser380 of human GCK.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using MBS616639.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using MBS616639.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of GCK in paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using MBS616639.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of GCK in paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using MBS616639.)
Immunocytochemistry (ICC)
(Immunocytochemical staining of NIH/3T3 cells, using MBS616639 in the presence (left) or absence (right) of GCK blocking peptide.)
Immunocytochemistry (ICC)
(Immunocytochemical staining of NIH/3T3 cells, using MBS616639 in the presence (left) or absence (right) of GCK blocking peptide.)
Related Product Information for anti-Germinal Center Kinase antibody
Germinal center kinase (GCK) is the founding member of the GCK family, a group of serine/threonine kinases homologous to the yeast Ste20 kinase. GCK is activated by TNF and associated with TRAF2 (TNF receptor-associated factor 2) and MEKK1, thereby activating the SAPK/JNK pathway (1,2). GCK does not significantly phosphorylate MEKK1, instead it enhances MEKK1 oligomerization and autophosphorylation (3). GCK binds and activates MLK3, indicating that GCK may have other effectors (3).
Product Categories/Family for anti-Germinal Center Kinase antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
germinal center kinase
Protein Family
Similar Products
Product Notes
The Germinal Center Kinase (Catalog #AAA616639) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Germinal Center Kinase (GC Kinase, GCK, B Lymphocyte Serine/threonine-protein Kinase, BL44, Mitogen-activated Protein Kinase Kinase Kinase Kinase 2, MAP4K2, MAPK/ERK Kinase Kinase Kinase 2, MEK Kinase Kinase 2, MEKKK 2, Rab8-interacting Protein, RAB8IP) reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Germinal Center Kinase can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF). Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunocytochemistry (IF): 1:100. Researchers should empirically determine the suitability of the Germinal Center Kinase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Germinal Center Kinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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