Rabbit anti-Human Synuclein, beta CT Polyclonal Antibody | anti-SNCB antibody
Synuclein, beta CT (Phosphoneuroprotein 14, PNP14)
Gene Names
SNCB; SNCB
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by antigen affinity chromatography.
Synonyms
Synuclein; beta CT; Polyclonal Antibody; beta CT (Phosphoneuroprotein 14; PNP14); Anti -Synuclein; anti-SNCB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Synuclein. The peptide sequence corresponding to human beta-synuclein (134 aa). Species sequence homology: rat beta-synuclein (137 aa) and bovine PNP14 (134 aa). It has no appreciable homology with synuclein-alpha or other proteins.
Purity/Purification
Affinity Purified
Purified by antigen affinity chromatography.
Purified by antigen affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Applicable Applications for anti-SNCB antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Western Blot: 1-10 ug/ml using Chemiluminescence technique
Dilution: ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well
Western Blot: 1-10 ug/ml using Chemiluminescence technique
Immunogen
Sunthetic 15 aa peptide located near the C-terminus of human synuclein-beta (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-SNCB antibody
Alzheimer's Disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and cognition in the elderly. One of the most important and initial step involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43aa peptides (beta amyloid1-40, 1-42, and 1-43). Polymerization of b-amyloid (Ab) and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition. Ab deposits have also been found to contain 2 additional proteins termed a-synuclein and b-synuclein. The 140 aa a-synucleins is identical with non-Ab component (NACP) of AD. The 134aa b-synuclein is homologous to 14kD bovine phosphoneuroprotein 14. Mutations in a-synuclein gene causing a replacement of alanine with a threonine has the potential to cause the protein to misfold. Synucleins are primarily expressed in the brain. At least 3 forms, two large (140aa SYN-1 & 149 aa SYN-2) and a small form (SYN-3, 42 aa) are produced by alternative splicing.
Product Categories/Family for anti-SNCB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
14,288 Da
NCBI Official Full Name
synuclein, beta
NCBI Official Synonym Full Names
synuclein, beta
NCBI Official Symbol
SNCB
NCBI Official Synonym Symbols
SNCB
NCBI Protein Information
beta-synuclein; OTTHUMP00000223369; OTTHUMP00000223370
UniProt Protein Name
Beta-synuclein
Protein Family
UniProt Gene Name
SNCB
UniProt Entry Name
SYUB_HUMAN
NCBI Description
The protein encoded by this gene is highly homologous to alpha-synuclein. These proteins are abundantly expressed in the brain and putatively inhibit phospholipase D2 selectively. The encoded protein, which may play a role in neuronal plasticity, is abundant in neurofibrillary lesions of patients with Alzheimer disease. This protein has been shown to be highly expressed in the substantia nigra of the brain, a region of neuronal degeneration in patients with Parkinson disease; however, no direct relation to Parkinson disease has been established. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
Uniprot Description
SNCB: a member of the synuclein family. Abundantly expressed in the brain. Inhibits phospholipase D2 selectively. May play a role in neuronal plasticity, is abundant in neurofibrillary lesions of Alzheimer's patients. Highly expressed in the substantia nigra of the brain, a region of neuronal degeneration in patients with Parkinson disease.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 5q35
Cellular Component: nucleoplasm; Golgi apparatus; growth cone; cell soma; mitochondrion; cytoplasm; terminal button; inclusion body
Molecular Function: phospholipase inhibitor activity; beta-tubulin binding; calcium ion binding; alpha-tubulin binding
Biological Process: synaptic transmission; negative regulation of catalytic activity; synapse organization and biogenesis; negative regulation of neuron apoptosis; dopamine metabolic process
Disease: Dementia, Lewy Body
Research Articles on SNCB
1. Gamma-synuclein protein is valuable for evaluation of progression of colorectal carcinoma; it is more sensitive to predict advanced stage and lymph node invasion when combined with either alpha- or beta-synuclein protein.
2. Our data confirm the fatty acid binding properties of alpha-syn, and to a lesser extent beta-syn, but suggest that gamma-syn does not share this same characteristic.
3. The aggregation behavior of alpha- and beta-synuclein as well as a series of chimeric variants were compared by exploring the structural transitions that occur in the presence of a widely used lipid mimetic, sodium dodecyl sulfate (SDS).
4. structural and functional properties of beta-synuclein were characterized using biochemical and bio-physical methods including: a functional assay, mass spectrometry, size exclusion chromatography, circular dichroism (CD), and fluorescence spectroscopy
5. Observational study of gene-disease association. (HuGE Navigator)
6. The accumulation of beta-synuclein was detectable only in the pons of Sandhoff disease cases. This differential accumulation of alpha- and beta-synucleins in human lipidoses may be related to functional differences between these two proteins.
7. A comparison of the structural and dynamic properties of the free states of all three synucleins, is reported in order to shed light on differences that may help to explain their different propensities to aggregate.
8. Of medulloblastomas, 76% have immunoreactivity for either alpha- or beta-synuclein or both; no immunoreactivity for gamma-synuclein is seen in medulloblastomas.
9. Biophysical properties of the synucleins and their propensities to fibrillate: inhibition of alpha-synuclein assembly by beta- and gamma-synucleins
10. An 11-residue deletion in the lipid-binding domain of beta-synuclein leads to the destabilization of an entire segment of the micelle-bound helical structure containing the deletion site.
2. Our data confirm the fatty acid binding properties of alpha-syn, and to a lesser extent beta-syn, but suggest that gamma-syn does not share this same characteristic.
3. The aggregation behavior of alpha- and beta-synuclein as well as a series of chimeric variants were compared by exploring the structural transitions that occur in the presence of a widely used lipid mimetic, sodium dodecyl sulfate (SDS).
4. structural and functional properties of beta-synuclein were characterized using biochemical and bio-physical methods including: a functional assay, mass spectrometry, size exclusion chromatography, circular dichroism (CD), and fluorescence spectroscopy
5. Observational study of gene-disease association. (HuGE Navigator)
6. The accumulation of beta-synuclein was detectable only in the pons of Sandhoff disease cases. This differential accumulation of alpha- and beta-synucleins in human lipidoses may be related to functional differences between these two proteins.
7. A comparison of the structural and dynamic properties of the free states of all three synucleins, is reported in order to shed light on differences that may help to explain their different propensities to aggregate.
8. Of medulloblastomas, 76% have immunoreactivity for either alpha- or beta-synuclein or both; no immunoreactivity for gamma-synuclein is seen in medulloblastomas.
9. Biophysical properties of the synucleins and their propensities to fibrillate: inhibition of alpha-synuclein assembly by beta- and gamma-synucleins
10. An 11-residue deletion in the lipid-binding domain of beta-synuclein leads to the destabilization of an entire segment of the micelle-bound helical structure containing the deletion site.
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Product Notes
The SNCB sncb (Catalog #AAA616575) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Synuclein, beta CT (Phosphoneuroprotein 14, PNP14) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Synuclein, beta CT can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well Western Blot: 1-10 ug/ml using Chemiluminescence technique. Researchers should empirically determine the suitability of the SNCB sncb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Synuclein, beta CT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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