Mouse MSX1 Monoclonal Antibody | anti-MSX1 antibody

MSX1 (msh Homeobox 1, HOX7, HYD1) (AP)

Cat. Num. AAA6164631

• Gene Names: MSX1; HOX7; HYD1; ECTD3; STHAG1
• Applications: ELISA, Immunoprecipitation, Western Blot
• Purity: Purified
• Synonyms: MSX1; Monoclonal Antibody; MSX1 (msh Homeobox 1; HOX7; HYD1) (AP); msh Homeobox 1; HYD1; anti-MSX1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 100
• Specificity: Recognizes MSX1.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-MSX1 antibody: ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: MSX1 (NP_002439, 216aa-297aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunoprecipitation (IP)

(Immunoprecipitation of MSX1 transfected lysate using anti-MSX1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with MSX1 MaxPab rabbit polyclonal antibody.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to MSX1 on HeLa cell. [antibody concentration 10 ug/ml])

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to MSX1 on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(MSX1 monoclonal antibody (M11), clone 1E2. Western Blot analysis of MSX1 expression in Raw 264.7.)

Western Blot (WB)

(MSX1 monoclonal antibody (M11), clone 1E2. Western Blot analysis of MSX1 expression in U-2 OS.)

Related Product Information for anti-MSX1 antibody

This gene encodes a member of the muscle segment homeobox gene family. The encoded protein functions as a transcriptional repressor during embryogenesis through interactions with components of the core transcription complex and other homeoproteins. It may also have roles in limb-pattern formation, craniofacial development, particularly odontogenesis, and tumor growth inhibition. Mutations in this gene, which was once known as homeobox 7, have been associated with nonsyndromic cleft lip with or without cleft palate 5, Witkop syndrome, Wolf-Hirschom syndrome, and autosomoal dominant hypodontia. [provided by RefSeq]

Product Categories/Family for anti-MSX1 antibody

Antibodies; Transcription Factors; Homeobox (HOX)

NCBI and Uniprot Product Information

• NCBI GI #: 118582284
• NCBI GeneID: 4487
• NCBI Accession #: NP_002439
• NCBI GenBank Nucleotide #: NM_002448
• UniProt Accession #: P28360
• Molecular Weight: 31kDa
• NCBI Official Full Name: homeobox protein MSX-1
• NCBI Official Synonym Full Names: msh homeobox 1
• NCBI Official Symbol: MSX1
• NCBI Official Synonym Symbols: HOX7; HYD1; ECTD3; STHAG1
• NCBI Protein Information: homeobox protein MSX-1
• UniProt Protein Name: Homeobox protein MSX-1
• Protein Family: Homeobox protein
• UniProt Gene Name: MSX1
• UniProt Synonym Gene Names: HOX7
• UniProt Entry Name: MSX1_HUMAN

NCBI Description

This gene encodes a member of the muscle segment homeobox gene family. The encoded protein functions as a transcriptional repressor during embryogenesis through interactions with components of the core transcription complex and other homeoproteins. It may also have roles in limb-pattern formation, craniofacial development, particularly odontogenesis, and tumor growth inhibition. Mutations in this gene, which was once known as homeobox 7, have been associated with nonsyndromic cleft lip with or without cleft palate 5, Witkop syndrome, Wolf-Hirschom syndrome, and autosomoal dominant hypodontia. [provided by RefSeq, Jul 2008]

Uniprot Description

MSX1: Acts as a transcriptional repressor. May play a role in limb-pattern formation. Acts in cranofacial development and specifically in odontogenesis. Expression in the developing nail bed mesenchyme is important for nail plate thickness and integrity. Expressed in the developing nail bed mesenchyme. Belongs to the Msh homeobox family.

Protein type: Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 4p16.2

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: p53 binding

Biological Process: embryonic forelimb morphogenesis; negative regulation of striated muscle cell differentiation; transcription from RNA polymerase II promoter; muscle development; protein stabilization; in utero embryonic development; regulation of odontogenesis; mesenchymal cell proliferation; cell morphogenesis; negative regulation of transcription from RNA polymerase II promoter; palate development; middle ear morphogenesis; embryonic hindlimb morphogenesis; odontogenesis of dentine-containing teeth; anterior/posterior pattern formation; negative regulation of cell proliferation; pituitary gland development; midbrain development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of cell growth; positive regulation of BMP signaling pathway; negative regulation of apoptosis

Disease: Orofacial Cleft 5; Witkop Syndrome; Tooth Agenesis, Selective, 1