Rabbit Ubiquitin Activating Enzyme, E1a Polyclonal Antibody | anti-Sae1 antibody

Ubiquitin Activating Enzyme, E1a (UBE 1a)

Cat. Num. AAA616112

• Gene Names: Sae1; AOS1; Sua1; Uble1a; HSPC140; AL033372; AW743391; D7Ertd177e; 2400010M20Rik; 2610044L12Rik
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Synonyms: Ubiquitin Activating Enzyme; E1a; Polyclonal Antibody; E1a (UBE 1a); Anti -Ubiquitin Activating Enzyme; anti-Sae1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Detects endogenous levels of total human UBE1a protein. Does not crossreact with UBE1b. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA and 50% glycerol.

• Applicable Applications for anti-Sae1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Immunofluorescence.; Dilution: Western blotting 1:1000; Immunoprecipitation 1:50; Immunohistochemistry (Paraffin) 1:175; IHC Protocol Citrate/TBST; Immunofluorescence (IF-IC) 1:50; Flow Cytometry 1:100
• Immunogen: Synthetic peptide (KLH-coupled) derived from the amino terminus of human UBE1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of extracts from K562 and SK-N-MC cells using MBS616112.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded glioblastoma using MBS616112.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse brain using MBS616112 in the presence of control peptide (left) or antigen specific peptide (right).)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of K562 cells using MBS616112(right) compared to a nonspecific negative control antibody (left).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of SK-N-MC cells using MBS616112 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).)

Related Product Information for anti-Sae1 antibody

Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the ubiquitin-activating enzyme (UBE1 or E1). The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the -amino group of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). UBE1 has two isofoms: UBE1a is a nuclear protein of 117kD while UBE1b is a nuclear and cytoplasmic protein of 110kD (5).

Product Categories/Family for anti-Sae1 antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 148710154
• NCBI GeneID: 56459
• UniProt Accession #: Q9R1T2; Q3TRG9; Q3TWJ0; Q9CSW9
• Molecular Weight: 38,620 Da
• NCBI Official Full Name: ubiquitin-like 1 (sentrin) activating enzyme E1A, isoform CRA_b
• NCBI Official Synonym Full Names: SUMO1 activating enzyme subunit 1
• NCBI Official Symbol: Sae1
• NCBI Official Synonym Symbols: AOS1; Sua1; Uble1a; HSPC140; AL033372; AW743391; D7Ertd177e; 2400010M20Rik; 2610044L12Rik
• NCBI Protein Information: SUMO-activating enzyme subunit 1; SUMO-1 activating enzyme subunit 1; ubiquitin-like 1-activating enzyme E1A; ubiquitin-like (sentrin) activating enzyme E1A; ubiquitin-like 1 (sentrin) activating enzyme E1A; ubiquitin-like 1 (sentrin) activating enzyme subunit 1
• UniProt Protein Name: SUMO-activating enzyme subunit 1
• Protein Family: SUMO-activating enzyme
• UniProt Gene Name: Sae1
• UniProt Synonym Gene Names: Aos1; Sua1; Ubl1a1; Uble1a
• UniProt Entry Name: SAE1_MOUSE

Uniprot Description

UBLE1A: The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. Belongs to the ubiquitin-activating E1 family.

Protein type: Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.19

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytosol; nucleus

Molecular Function: protein C-terminus binding; SUMO activating enzyme activity; ATP-dependent protein binding; small protein activating enzyme activity; protein heterodimerization activity; ligase activity

Biological Process: protein sumoylation; regulation of mitotic cell cycle; protein modification process