Mouse anti-Human TRIM25 Monoclonal Antibody | anti-TRIM25 antibody

TRIM25 (E3 Ubiquitin/ISG15 Ligase TRIM25, Estrogen-responsive Finger Protein, EFP, RNF147, ZNF147, RING Finger Protein 147, Tripartite Motif-containing Protein 25, Ubiquitin/ISG15-conjugating Enzyme TRIM25, Zinc Finger Protein 147) (PE)

Cat. Num. AAA6160831

• Gene Names: TRIM25; EFP; Z147; RNF147; ZNF147
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TRIM25; Monoclonal Antibody; TRIM25 (E3 Ubiquitin/ISG15 Ligase TRIM25; Estrogen-responsive Finger Protein; EFP; RNF147; ZNF147; RING Finger Protein 147; Tripartite Motif-containing Protein 25; Ubiquitin/ISG15-conjugating Enzyme TRIM25; Zinc Finger Protein 147) (PE); anti-TRIM25 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 5C3
• Specificity: Recognizes human TRIM25.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TRIM25 antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 20ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa211-311 from human TRIM25 (NP_005073) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: SQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDEFEFLEK*
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (37.11kD))

Western Blot (WB)

(TRIM25 monoclonal antibody Western Blot analysis of TRIM25 expression in Hela NE)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to TRIM25 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 3ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to TRIM25 on HeLa cell. [antibody concentration 20ug/ml])

Product Categories/Family for anti-TRIM25 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 68160937
• NCBI GeneID: 7706
• NCBI Accession #: NP_005073
• NCBI GenBank Nucleotide #: NM_005082
• UniProt Accession #: Q14258
• Molecular Weight: 70,973 Da
• NCBI Official Full Name: E3 ubiquitin/ISG15 ligase TRIM25
• NCBI Official Synonym Full Names: tripartite motif containing 25
• NCBI Official Symbol: TRIM25
• NCBI Official Synonym Symbols: EFP; Z147; RNF147; ZNF147
• NCBI Protein Information: E3 ubiquitin/ISG15 ligase TRIM25; RING finger protein 147; zinc finger protein-147; tripartite motif-containing 25; tripartite motif protein TRIM25; estrogen-responsive finger protein; tripartite motif-containing protein 25; ubiquitin/ISG15-conjugating en
• UniProt Protein Name: E3 ubiquitin/ISG15 ligase TRIM25
• Protein Family: E3 ubiquitin/ISG15 ligase
• UniProt Gene Name: TRIM25
• UniProt Synonym Gene Names: EFP; RNF147; ZNF147
• UniProt Entry Name: TRI25_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TRIM25: a ubiquitous protein that mediates estrogen action in various target organs. Contains one RING-type zinc finger and one SPRY domain.

Protein type: EC 6.3.2.n3; Ubiquitin conjugating system; Ubiquitin ligase; Nuclear receptor co-regulator; Transcription factor; Ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 17q23.2

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: protein binding; acid-amino acid ligase activity; zinc ion binding; transcription factor activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; negative regulation of virion penetration into host cell; cytokine and chemokine mediated signaling pathway; protein ubiquitination; regulation of virion penetration into host cell; activation of NF-kappaB transcription factor; response to vitamin D; response to estrogen stimulus; innate immune response; positive regulation of transcription factor activity; negative regulation of interferon type I production; defense response to virus