Rabbit anti-Rat Anion Exchanger 1 Polyclonal Antibody | anti-SLC4A1 antibody
Anion Exchanger 1 (AE1)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Western Blot: 1-10ug/ml (ECL.) AE1 has a molecular weight of ~90-100kD. It aggregates easily forming dimers and multimers. It may also be proteolytically processed to smaller fragments of 60, 40 and 20kD.
ELISA: 1-10ug/ml. Using 1ug/ml A2295-13B4 control peptide per well.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system. Southeast Asian ovalocytosis (SAO, Melanesian ovalocytosis) results from the heterozygous presence of a deletion in the encoded protein and is common in areas where Plasmodium falciparum malaria is endemic. One null mutation in this gene is known, resulting in very severe anemia and nephrocalcinosis. [provided by RefSeq]
Uniprot Description
SLC4A1: Band 3 is the major integral glycoprotein of the erythrocyte membrane. Band 3 has two functional domains. Its integral domain mediates a 1:1 exchange of inorganic anions across the membrane, whereas its cytoplasmic domain provides binding sites for cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Defects in SLC4A1 are the cause of elliptocytosis type 4 (EL4). EL4 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape. Defects in SLC4A1 are the cause of spherocytosis type 4 (SPH4); also known as hereditary spherocytosis type 4 (HS4). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. Defects in SLC4A1 are the cause of renal tubular acidosis, distal, autosomal dominant (AD-dRTA). A disease characterized by reduced ability to acidify urine, variable hyperchloremic hypokalemic metabolic acidosis, nephrocalcinosis, and nephrolithiasis. Defects in SLC4A1 are the cause of renal tubular acidosis, distal, with hemolytic anemia (dRTA-HA). A disease characterized by the association of hemolytic anemia with distal renal tubular acidosis, the reduced ability to acidify urine resulting in variable hyperchloremic hypokalemic metabolic acidosis, nephrocalcinosis, and nephrolithiasis. Defects in SLC4A1 are the cause of renal tubular acidosis, distal, with normal red cell morphology (dRTA-NRC). A disease characterized by reduced ability to acidify urine, variable hyperchloremic hypokalemic metabolic acidosis, nephrocalcinosis, and nephrolithiasis. Belongs to the anion exchanger (TC 2.A.31) family.
Protein type: Transporter; Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 17q21.31
Cellular Component: cortical cytoskeleton; basolateral plasma membrane; integral to plasma membrane; plasma membrane; integral to membrane; Z disc
Molecular Function: protein C-terminus binding; bicarbonate transmembrane transporter activity; protein binding; protein homodimerization activity; protein anchor; chloride transmembrane transporter activity; inorganic anion exchanger activity; anion:anion antiporter activity; anion transmembrane transporter activity; ankyrin binding; actin binding
Biological Process: cellular ion homeostasis; bicarbonate transport; regulation of intracellular pH; chloride transport; ion transport; transmembrane transport; anion transport
Disease: Blood Group--swann System; Blood Group--froese; Renal Tubular Acidosis, Distal, Autosomal Dominant; Blood Group--wright Antigen; Renal Tubular Acidosis, Distal, With Hemolytic Anemia; Spherocytosis, Type 4; Blood Group--diego System; Malaria, Susceptibility To; Blood Group--waldner Type
Research Articles on SLC4A1
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Product Notes
The SLC4A1 slc4a1 (Catalog #AAA615456) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anion Exchanger 1 (AE1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Anion Exchanger 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot and ELISA. Dilution: Western Blot: 1-10ug/ml (ECL.) AE1 has a molecular weight of ~90-100kD. It aggregates easily forming dimers and multimers. It may also be proteolytically processed to smaller fragments of 60, 40 and 20kD. ELISA: 1-10ug/ml. Using 1ug/ml A2295-13B4 control peptide per well. Researchers should empirically determine the suitability of the SLC4A1 slc4a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Anion Exchanger 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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