Mouse anti-Human HSD3B1 Monoclonal Antibody | anti-HSD3B1 antibody

HSD3B1 (3 beta-hydroxysteroid Dehydrogenase/Delta 5->4-isomerase Type 1, 3 beta-hydroxysteroid Dehydrogenase/Delta 5->4-isomerase Type I, 3-beta-HSD I, Trophoblast Antigen FDO161G, 3BH, HSDB3A) (HRP)

Cat. Num. AAA6152969

• Gene Names: HSD3B1; I; HSD3B; HSDB3; HSDB3A; SDR11E1; 3BETAHSD
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HSD3B1; Monoclonal Antibody; HSD3B1 (3 beta-hydroxysteroid Dehydrogenase/Delta 5->4-isomerase Type 1; 3 beta-hydroxysteroid Dehydrogenase/Delta 5->4-isomerase Type I; 3-beta-HSD I; Trophoblast Antigen FDO161G; 3BH; HSDB3A) (HRP); anti-HSD3B1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3C11-D4
• Specificity: Recognizes human HSD3B1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-HSD3B1 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 3ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-373 from human HSD3B1 (AAH31999.1) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MTGWSCLVTGAGGFLGQRIIRLLVKEKELKEIRVLDKAFGPELREEFSKLQNKTKLTVLEGDILDEPFLKRACQDVSVIIHTACIIDVFGVTHRESIMNVNVKGTQLLLEACVQASVPVFIYTSSIEVAGPNSYKEIIQNGHEEEPLENTWPAPYPHSKKLAEKAVLAANGWNLKNGGTLYTCALRPMYIYGEGSRFLSASINEALNNNGILSSVGKFSTVNPVYVGNVAWAHILALRALQDPKKAPSIRGQFYYISDDTPHQSYDNLNYTLSKEFGLRLDSRWSFPLSLMYWIGFLLEIVSFLLRPIYTYRPPFNRHIVTLSNSVFTFSYKKAQRDLAYKPLYSWEEAKQKTVEWVGSLVDRHKENLKSKTQ
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (66.77kD).)

Western Blot (WB)

(HSD3B1 monoclonal antibody Western Blot analysis of HSD3B1 expression in human placenta.)

Western Blot (WB)

(Western Blot analysis of HSD3B1 expression in transfected 293T cell line by HSD3B1 monoclonal antibody Lane 1: HSD3B1 transfected lysate (Predicted MW: 42.3kD. Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to HSD3B1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml])

Immunohistochemistry (IHC)

(Immunohistochemical staining of various tissues with hematoxylin and HSD3B1 antibody under high magnification. (a) Stomach (b) Esophagus (c) Endometrium (d) Uterine cervix (e) Placenta (f) Ovary, clear cell carcinoma (g [antibody concentration 3ug/ml])

Product Categories/Family for anti-HSD3B1 antibody

Antibodies; Enzymes; Dehydrogenase

References

1. Gestational Trophoblastic Tumors and Related Tumor-Like Lesions. Shih IM, Mazur MT, Kurman RJ.Blaustein's Pathology of the Female Genital Tract 2011, 1075-1135, DOI: 10.1007/978 -1-4419-0489-8_20 2. Advances in the diagnosis of gestational trophoblastic tumors and tumor-like lesions. Mao TL, Shih IM.Expert Opin. Med. Diagn. (2009) 3(4):371-380. 3. Chorangiocarcinoma: A Case Report and Review of the Literature. Ariel I, Boldes R, Weintraub A, Reinus C, Beller U, Arbel R.Int J Gynecol Pathol. 2009 May;28(3):267-71. 4. HSD3B1 as a novel trophoblast-associated marker that assists in the differential diagnosis of trophoblastic tumors and tumorlike lesions. Mao TL, Kurman RJ, Jeng YM, Huang W, Shih IM.Am J Surg Pathol. 2008 Feb;32(2):236-42. 5. Trophogram, an immunohistochemistry-based algorithmic approach, in the differential diagnosis of trophoblastic tumors and tumorlike lesions. Shih IeM.Ann Diagn Pathol. 2007 Jun;11(3):228-34.

NCBI and Uniprot Product Information

• NCBI GI #: 21619293
• NCBI GeneID: 3283
• Molecular Weight: 42,252 Da
• NCBI Official Full Name: Homo sapiens hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1, mRNA
• NCBI Official Synonym Full Names: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
• NCBI Official Symbol: HSD3B1
• NCBI Official Synonym Symbols: I; HSD3B; HSDB3; HSDB3A; SDR11E1; 3BETAHSD
• NCBI Protein Information: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
• Protein Family: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase

NCBI Description

The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids. [provided by RefSeq, Jun 2016]