Mouse anti-Human HOMER1 Monoclonal Antibody | anti-HOMER1 antibody

HOMER1 (Homer Protein Homolog 1, Homer-1, SYN47) (HRP)

Cat. Num. AAA6152923

• Gene Names: HOMER1; HOMER; SYN47; Ves-1; HOMER1A; HOMER1B; HOMER1C
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HOMER1; Monoclonal Antibody; HOMER1 (Homer Protein Homolog 1; Homer-1; SYN47) (HRP); anti-HOMER1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 2F8
• Specificity: Recognizes human HOMER1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-HOMER1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full-length recombinant corresponding to aa1-354 from HOMER1 (AAH15502) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MGEQPIFSTRAHVFQIDPNTKKNWVPTSKHAVTVSYFYDSTRNVYRIISLDGSKAIINSTITPNMTFTKTSQKFGQWADSRANTVYGLGFSSEHHLSKFAEKFQEFKEAARLAKEKSQEKMELTSTPSQESAGGDLQSPLTPESINGTDDERTPDVTQNSEPRAEPTQNALPFSHSSAISKHWEAELATLKGNNAKLTAALLESTANVKQWKQQLAAYQEEAERLHKRVTELECVSSQANAVHTHKTELNQTIQELEETLKLKEEEIERLKQEIDNARELQEQRDSLTQKLQEVEIRNKDLEGQLSDLEQRLEKSQNEQEAFRNNLKTLLEILDGKIFELTELRDNLAKLLECS
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of HOMER1 expression in transfected 293T cell line by HOMER1 monoclonal antibody Lane 1: HOMER1 transfected lysate (40.3kD). Lane 2: Non-transfected lysate. )

Testing Data

(Detection limit for recombinant GST tagged HOMER1 is 1ng/ml as a capture antibody.)

Related Product Information for anti-HOMER1 antibody

Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development.

Product Categories/Family for anti-HOMER1 antibody

Antibodies; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 15930121
• NCBI GeneID: 9456
• Molecular Weight: 19,861 Da
• NCBI Official Full Name: Homo sapiens homer homolog 1 (Drosophila), mRNA
• NCBI Official Synonym Full Names: homer scaffolding protein 1
• NCBI Official Symbol: HOMER1
• NCBI Official Synonym Symbols: HOMER; SYN47; Ves-1; HOMER1A; HOMER1B; HOMER1C
• NCBI Protein Information: homer protein homolog 1
• Protein Family: Homer protein

NCBI Description

This gene encodes a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function. [provided by RefSeq, Jul 2008]