Mouse anti-Human DCX Monoclonal Antibody | anti-DCX antibody

DCX (Neuronal Migration Protein Doublecortin, Lissencephalin-X, Lis-X, Doublin, DBCN, LISX) (HRP)

Cat. Num. AAA6152095

• Gene Names: DCX; DC; DBCN; LISX; SCLH; XLIS
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DCX; Monoclonal Antibody; DCX (Neuronal Migration Protein Doublecortin; Lissencephalin-X; Lis-X; Doublin; DBCN; LISX) (HRP); anti-DCX antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1G12
• Specificity: Recognizes human DCX.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-DCX antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-360 from human DCX (AAH27925) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MELDFGHFDERDKTSRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQVLTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKDLYLPLSLDDSDSLGDSM
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (65.34kD).)

Western Blot (WB)

(Western Blot analysis of DCX expression in transfected 293T cell line by DCX monoclonal antibody. Lane 1: DCX transfected lysate (41.4kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to DCX on HeLa cell. [antibody concentration 10ug/ml].)

Related Product Information for anti-DCX antibody

In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. DCX is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. It contains two doublecortin domains, which bind microtubules. In addition, this protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in the gene encoding DCX are a cause of X-linked lissencephaly.

Product Categories/Family for anti-DCX antibody

Antibodies; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 20381232
• NCBI GeneID: 1641
• Molecular Weight: 40,044 Da
• NCBI Official Full Name: Homo sapiens doublecortin, mRNA
• NCBI Official Synonym Full Names: doublecortin
• NCBI Official Symbol: DCX
• NCBI Official Synonym Symbols: DC; DBCN; LISX; SCLH; XLIS
• NCBI Protein Information: neuronal migration protein doublecortin
• Protein Family: Protein doublecortin

NCBI Description

This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]