anti-Mouse Period Clock Protein 2 Polyclonal Antibody
Anti - Period Clock Protein 2 (PER2)
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
Period Clock Protein 2; Polyclonal Antibody; Anti - Period Clock Protein 2 (PER2); Period Clock Protein 2 (PER2); anti-Period Clock Protein 2 antibody
Host
Host: Rabbit
Source: Mouse
Source: Mouse
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
The mouse peptide sequence is 77% conserved in rat Per2. No significant homology is seen with human Per2 or Per1 or Per3. Antibody crossreactivity with Per2 from other species is not known. Control peptide, because of its low MW (<3kD), is not suitable for Western. It is recommed for ELISA, dot blot, or for antibody blocking experiments (use 5-10 ug of control peptide per 1 ug of aff pure IgG or 1 ul of antiserum) to demonstrate antibody specificity
Purity/Purification
Serum
Form/Format
Serum
Supplied as a liquid, neat serum, 40% glycerol.
Supplied as a liquid, neat serum, 40% glycerol.
Applicable Applications for anti-Period Clock Protein 2 antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
ELISA: ~1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
ELISA: ~1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Immunogen
A 23-aa peptide sequence within C-terminus of mouse Per2 (1) was synthesized coupled to KLH
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Product Categories/Family for anti-Period Clock Protein 2 antibody
References
• :(1). Albretch U et al (1997) Cell 91, 1055-1064; Shearman LP et al (1997) Neuron 19, 1261-1269; Sakamoto K et al (1998) J Biol. Chem. 273, 27039-27342.
• 2. Nagase T eat al (1997) DNA Res. 4, 141-150
• 3. Field MD et al (2000) Neuron 25, 437; Bae K et al (2000) 30, 525-536; Nuesslein-Hildesheim B et al (2000) Eur. J. Neurol. 2856.
• 2. Nagase T eat al (1997) DNA Res. 4, 141-150
• 3. Field MD et al (2000) Neuron 25, 437; Bae K et al (2000) 30, 525-536; Nuesslein-Hildesheim B et al (2000) Eur. J. Neurol. 2856.
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Product Notes
The Period Clock Protein 2 (Catalog #AAA614750) is an Antibody produced from Host: Rabbit Source: Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti - Period Clock Protein 2 (PER2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Period Clock Protein 2 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA, Western Blotting. Other applications not tested. Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. ELISA: ~1:100,000 using 50-100ng control peptide/well. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Period Clock Protein 2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Period Clock Protein 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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