Mouse anti-Human FRG1 Monoclonal Antibody | anti-FRG1 antibody

FRG1 (FSHD Region Gene 1 Protein, FRG1A, FSG1, Protein FRG1) (FITC)

Cat. Num. AAA6147271

• Gene Names: FRG1; FSG1; FRG1A
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FRG1; Monoclonal Antibody; FRG1 (FSHD Region Gene 1 Protein; FRG1A; FSG1; Protein FRG1) (FITC); anti-FRG1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 4A5
• Specificity: Recognizes human FRG1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-FRG1 antibody: ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IF: 35ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-258 from human FRG1 (AAH53997) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MAEYSYVKSTKLVLKGTKTKSKKKKSKDKKRKREEDEETQLDIVGIWWTVTNFGEISGTIAIEMDKGTYIHALDNGLFTLGAPHKEVDEGPSPPEQFTAVKLSDSRIALKSGYGKYLGINSDGLVVGRSDAIGPREQWEPVFQNGKMALLASNSCFIRCNEAGDIEAKSKTAGEEEMIKIRSCAERETKKKDDIPEEDKGNVKQCEINYVKKFQSFQDHKLKISKEDSKILKKARKDGFLHETLLDRRAKLKADRYCK
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (54.12kD).)

Western Blot (WB)

(FRG1 monoclonal antibody, Western Blot analysis of FRG1 expression in HeLa NE.)

Western Blot (WB)

(Western Blot analysis of FRG1 expression in transfected 293T cell line by FRG1 monoclonal antibody. Lane 1: FRG1 transfected lysate (29kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to FRG1 on HeLa cell. [antibody concentration 35ug/ml].)

Immunoprecipitation (IP)

(Immunoprecipitation of FRG1 transfected lysate using FRG1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with FRG1 rabbit polyclonal antibody.)

Product Categories/Family for anti-FRG1 antibody

Antibodies; DNA/RNA Translation

References

1. RNA Interference Improves Myopathic Phenotypes in Mice Over-expressing FSHD Region Gene 1 (FRG1). Wallace LM, Garwick-Coppens SE, Tupler R, Harper SQ.Mol Ther. 2011 Jul 5. doi: 10.1038/mt.2011.118.

NCBI and Uniprot Product Information

• NCBI GI #: 32450692
• NCBI GeneID: 2483
• Molecular Weight: 29,172 Da
• NCBI Official Full Name: Homo sapiens FSHD region gene 1, mRNA
• NCBI Official Synonym Full Names: FSHD region gene 1
• NCBI Official Symbol: FRG1
• NCBI Official Synonym Symbols: FSG1; FRG1A
• NCBI Protein Information: protein FRG1
• Protein Family: Protein

NCBI Description

This gene maps to a location 100 kb centromeric of the repeat units on chromosome 4q35 which are deleted in facioscapulohumeral muscular dystrophy (FSHD). It is evolutionarily conserved and has related sequences on multiple human chromosomes but DNA sequence analysis did not reveal any homology to known genes. In vivo studies demonstrate the encoded protein is localized to the nucleolus. [provided by RefSeq, Jul 2008]