Rabbit anti-Human Podocalyxin Polyclonal Antibody | anti-PODXL antibody

Podocalyxin

Cat. Num. AAA614411

• Gene Names: PODXL; PC; PCLP; Gp200; PCLP-1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Podocalyxin; Polyclonal Antibody; Anti -Podocalyxin; anti-PODXL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Podocalyxin. Species Sequence Homology: mouse, rat - 93%.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, before the addition of glycerol to 40%.

• Applicable Applications for anti-PODXL antibody: ELISA (EL/EIA), Western Blot (WB)
• Immunogen: Synthetic15aa peptide corresponding to the ~C-terminus of human Podocalyxin (KLH).
• Preparation and Storage: 4 degree C (-20 degree C Glycerol)

Related Product Information for anti-PODXL antibody

Podocalyxin, a 485aa protein in rat and 528aa in human (chr 7q32-q33). Functions as an antiadhesin that maintains an open filtration pathway between neighboring foot processes in the podocytes by charge repulsion. Mainly expressed in Glomerular epithelium cells. Nephrin is encoded by NPHS1 gene (chromosome 19q13.1), the core protein of the interpodocytes slit diaphragm of kidney glomerulus, these podocytes are highly differentiated with characteristic interdigitating foot processes covering the outer glomerular basement membrane, the space between these foot processes is spanned by a tight junction to provide the large surface area for filtration. The mutated NPHS1 gene in congenital nephrotic syndrome of Finnish type, a human disease that leads to massive proteinuria in utero and nephrosis at birth. Nephrin is a transmembrane protein of immunoglobulin super family with 8 Ig like domains, identified in human (1241aa), rat (1252aa), and mouse (1256aa). Nephrin is found to be specifically expressed in kidney-slit diaphragm, brain and pancreas, the protein plays a crucial role in development and function of kidney filtration barrier, this barrier is crucial for maintaining the water and electrolyte balance without losing circulating proteins into the urine. Nephrin interacts with podocin and with CD2AP C-terminal domain, It tends to be a signaling molecule that activates canonical protein kinase cascades, which is initiated by three closely related proteins called NEPH proteins (NEPH1, NEPH2, & NEPH3), The NEPH proteins share a common domain architecture consisting of 5 extracellular Ig domain followed by transmembrane domain, These proteins bind to C-terminal domain of podocin, which interacts with C-terminal of nephrin and greatly enhances nephrin-induced signaling.

Product Categories/Family for anti-PODXL antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 66277202
• NCBI GeneID: 5420
• NCBI Accession #: NP_001018121.1
• NCBI GenBank Nucleotide #: NM_001018111.2
• UniProt Accession #: O00592; Q52LZ7; Q53ER6; A6NHX8
• Molecular Weight: 58,635 Da
• NCBI Official Full Name: podocalyxin isoform 1
• NCBI Official Synonym Full Names: podocalyxin-like
• NCBI Official Symbol: PODXL
• NCBI Official Synonym Symbols: PC; PCLP; Gp200; PCLP-1
• NCBI Protein Information: podocalyxin; GCTM-2 antigen; podocalyxin-like protein 1
• UniProt Protein Name: Podocalyxin
• Protein Family: Podocalyxin
• UniProt Gene Name: PODXL
• UniProt Synonym Gene Names: PCLP; PCLP1; PC; PCLP-1
• UniProt Entry Name: PODXL_HUMAN

NCBI Description

This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]

Uniprot Description

PODXL: Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. Belongs to the podocalyxin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 7q32-q33

Cellular Component: ruffle; extracellular space; integral to plasma membrane; lamellipodium; microvillus membrane; apical plasma membrane; cytoplasm; plasma membrane; lipid raft; filopodium

Molecular Function: protein binding

Biological Process: cell migration; negative regulation of cell adhesion; negative regulation of cell-cell adhesion; cell adhesion; leukocyte migration; positive regulation of cell-cell adhesion mediated by integrin; regulation of microvillus biogenesis; positive regulation of cell migration