Mouse anti-Human PSMB6 Monoclonal Antibody | anti-PSMB6 antibody

PSMB6 (Proteasome Subunit beta Type-6, Proteasome delta Chain, Macropain delta Chain, Multicatalytic Endopeptidase Complex delta Chain, Proteasome Subunit Y, LMPY) (Biotin)

Cat. Num. AAA6143792

• Gene Names: PSMB6; Y; LMPY; DELTA
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PSMB6; Monoclonal Antibody; PSMB6 (Proteasome Subunit beta Type-6; Proteasome delta Chain; Macropain delta Chain; Multicatalytic Endopeptidase Complex delta Chain; Proteasome Subunit Y; LMPY) (Biotin); anti-PSMB6 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: S51
• Specificity: Recognizes human PSMB6.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 834

• Applicable Applications for anti-PSMB6 antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 10ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to human PSMB6, aa1-240 (AAH00835) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MAATLLAARGAGPAPAWGPEAFTPDWESREVSTGTTIMAVQFDGGVVLGADSRTTTGSYIANRVTDKLTPIHDRIFCCRSGSAADTQAVADAVTYQLGFHSIELNEPPLVHTAASLFKEMCYRYREDLMAGIIIAGWDPQEGGQVYSVPMGGMMVRQSFAIGGSGSSYIYGYVDATYREGMTKEECLQFTANALALAMERDGSSGGVIRLAAIAESGVERQVLLGDQIPKFAVATLPPA
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PSMB6 monoclonal antibody Western Blot analysis of PSMB6 expression in A-431.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to PSMB6 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1ug/ml])

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to PSMB6 on HeLa cell. [antibody concentration 10ug/ml])

Testing Data

(Detection limit for recombinant GST tagged PSMB6 is ~0.3ng/ml as a capture antibody.)

Product Categories/Family for anti-PSMB6 antibody

Antibodies; Proteasomes

NCBI and Uniprot Product Information

• NCBI GI #: 34783069
• NCBI GeneID: 5694
• NCBI Official Full Name: Homo sapiens proteasome (prosome, macropain) subunit, beta type, 6, mRNA
• NCBI Official Synonym Full Names: proteasome 20S subunit beta 6
• NCBI Official Symbol: PSMB6
• NCBI Official Synonym Symbols: Y; LMPY; DELTA
• NCBI Protein Information: proteasome subunit beta type-6
• Protein Family: Proteasome

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]