Mouse anti-Human PPP2R1A Monoclonal Antibody | anti-PPP2R1A antibody

PPP2R1A (Serine/Threonine-Protein Phosphatase 2A 65kD Regulatory Subunit A alpha Isoform, PP2A Subunit A Isoform PR65-alpha, PP2A Subunit A Isoform R1-alpha, Medium Tumor Antigen-associated 61kD Protein, MGC786) (Biotin)

Cat. Num. AAA6143706

• Gene Names: PPP2R1A; PR65A; PP2AAALPHA; PP2A-Aalpha
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PPP2R1A; Monoclonal Antibody; PPP2R1A (Serine/Threonine-Protein Phosphatase 2A 65kD Regulatory Subunit A alpha Isoform; PP2A Subunit A Isoform PR65-alpha; PP2A Subunit A Isoform R1-alpha; Medium Tumor Antigen-associated 61kD Protein; MGC786) (Biotin); anti-PPP2R1A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4E6
• Specificity: Recognizes human PPP2R1A.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PPP2R1A antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-589 from human PPP2R1A (AAH01537) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MAAADGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRSELLPFLTDTIYDEDEVLLALAEQLGTFATLVGGPEYVHCLLPPLESLATVEETVVRDKAVESLRAISHEHSPSDLEAHFVPLVKRLAGGDWFTSRTSACGLFSVCYPRVSSAVKAELRQYFRNLCSDDTPMVRRAAASKLGEFAKVLELDNVKSEIIPMFSNLASDEQDSVRLLAVEACVNIAQLLPQEDLEALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCRENVIMSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKYFAQEALTVLSLA
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PPP2R1A monoclonal antibody Western Blot analysis of PPP2R1A expression in Jurkat.)

Testing Data

(Detection limit for recombinant GST tagged PPP2R1A is 3ng/ml as a capture antibody.)

Testing Data

(Proximity Ligation Analysis of protein-protein interactions between TP53 and PPP2R1A HeLa cells were stained with TP53 rabbit purified polyclonal 1:1200 and PPP2R1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Related Product Information for anti-PPP2R1A antibody

PPP2R1A is a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.

Product Categories/Family for anti-PPP2R1A antibody

Antibodies; Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 34783905
• NCBI GeneID: 5518
• Molecular Weight: 65,309 Da
• NCBI Official Full Name: Homo sapiens protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform, mRNA
• NCBI Official Synonym Full Names: protein phosphatase 2, regulatory subunit A, alpha
• NCBI Official Symbol: PPP2R1A
• NCBI Official Synonym Symbols: PR65A; PP2AAALPHA; PP2A-Aalpha
• NCBI Protein Information: serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha; medium tumor antigen-associated 61 KDA protein; protein phosphatase 2 (formerly 2A), regulatory subunit

NCBI Description

This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]