Chicken anti-Human CARD14, Isoform 2 Polyclonal Antibody | anti-CARD14 antibody

CARD14, Isoform 2 (Caspase Recruitment Domain, CARD-containing MAGUK 2)

Cat. Num. AAA613778

• Gene Names: CARD14; BIMP2; CARMA2
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CARD14; Isoform 2; Polyclonal Antibody; Isoform 2 (Caspase Recruitment Domain; CARD-containing MAGUK 2); Anti -CARD14; anti-CARD14 antibody

• Host: Chicken
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: Recognizes human Caspase Recruitment Domain Protein 14 Isoform 2.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Glycerol free.

• Applicable Applications for anti-CARD14 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000-1:2000
• Immunogen: Synthetic peptide corresponding to aa221-330
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.)

Product Categories/Family for anti-CARD14 antibody

Antibodies; Abs to Apoptosis; Caspase

NCBI and Uniprot Product Information

• NCBI GI #: 16507955
• NCBI GeneID: 79092
• NCBI Accession #: NP_438170.1
• NCBI GenBank Nucleotide #: NM_052819.2
• UniProt Accession #: Q9BXL6; Q9BVB5
• Molecular Weight: 113,270 Da
• NCBI Official Full Name: caspase recruitment domain-containing protein 14 isoform 2
• NCBI Official Synonym Full Names: caspase recruitment domain family, member 14
• NCBI Official Symbol: CARD14
• NCBI Official Synonym Symbols: BIMP2; CARMA2
• NCBI Protein Information: caspase recruitment domain-containing protein 14; carma 2; card-maguk protein 2; CARD-containing MAGUK 2 protein; CARD-containing MAGUK protein 2; bcl10-interacting maguk protein 2
• UniProt Protein Name: Caspase recruitment domain-containing protein 14
• Protein Family: Caspase recruitment domain-containing protein
• UniProt Gene Name: CARD14
• UniProt Synonym Gene Names: CARMA2
• UniProt Entry Name: CAR14_HUMAN

NCBI Description

The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein shares a similar domain structure with CARD11 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10. Two alternatively spliced variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]

Uniprot Description

CARD14: a protein containing a caspase recruitment domain (CARD) that functions as an activator of BCL10 and NF-kappaB signaling. CARD is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in signaling through highly specific protein-protein homophilic interactions. CARD proteins function as scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. The CARD domain of CARD14 associates specifically with the CARD domains of CARD10 and BCL10, a signaling protein that activates NF-kB through the IkappaB kinase complex in response to upstream stimuli. When expressed in cells, CARD14 activates NF-kappaB and induces the phosphorylation of BCL10. A subgroup of primary lymphomas of the central nervous system have higher levels of CARD14 expression.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: cytoplasm; plasma membrane

Molecular Function: CARD domain binding

Biological Process: tumor necrosis factor-mediated signaling pathway; apoptosis; activation of NF-kappaB-inducing kinase; positive regulation of protein amino acid phosphorylation; negative regulation of apoptosis; activation of NF-kappaB transcription factor

Disease: Psoriasis 2; Pityriasis Rubra Pilaris