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Western Blot (WB) (Western Blot detection against Immunogen (36.74kD).)

Mouse anti-Human SERPINB1 Monoclonal Antibody | anti-SERPINB1 antibody

SERPINB1 (Leukocyte Elastase Inhibitor, LEI, Monocyte/Neutrophil Elastase Inhibitor, EI, M/NEI, Peptidase Inhibitor 2, PI-2, Serpin B1, ELANH2, MNEI, PI2) (AP)

Gene Names
SERPINB1; EI; LEI; PI2; MNEI; PI-2; HEL57; M/NEI; ELANH2
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SERPINB1; Monoclonal Antibody; SERPINB1 (Leukocyte Elastase Inhibitor; LEI; Monocyte/Neutrophil Elastase Inhibitor; EI; M/NEI; Peptidase Inhibitor 2; PI-2; Serpin B1; ELANH2; MNEI; PI2) (AP); anti-SERPINB1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
4D7
Specificity
Recognizes human SERPINB1.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-SERPINB1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa201-300 from human SERPINB1 (NP_109591.1) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
KKKFAYGYIEDLKCRVLELPYQGEELSMVILLPDDIEDESTGLKKIEEQLTLEKLHEWTKPENLDFIEVNVSLPRFKLEESYTLNSDLARLGVQDLFNSS
Conjugate
AP
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (36.74kD).)

Western Blot (WB) (Western Blot detection against Immunogen (36.74kD).)

Western Blot (WB)

(Western Blot analysis of SERPINB1 expression in transfected 293T cell line by SERPINB1 monoclonal antibody. Lane 1: SERPINB1 transfected lysate (42.7kD). Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of SERPINB1 expression in transfected 293T cell line by SERPINB1 monoclonal antibody. Lane 1: SERPINB1 transfected lysate (42.7kD). Lane 2: Non-transfected lysate.)

Testing Data

(Detection limit for recombinant GST tagged SERPINB1 is 0.3ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged SERPINB1 is 0.3ng/ml as a capture antibody.)
Related Product Information for anti-SERPINB1 antibody
Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3.
Product Categories/Family for anti-SERPINB1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,742 Da
NCBI Official Full Name
leukocyte elastase inhibitor
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade B (ovalbumin), member 1
NCBI Official Symbol
SERPINB1
NCBI Official Synonym Symbols
EI; LEI; PI2; MNEI; PI-2; HEL57; M/NEI; ELANH2
NCBI Protein Information
leukocyte elastase inhibitor; peptidase inhibitor 2; epididymis luminal protein 57; protease inhibitor 2 (anti-elastase), monocyte/neutrophil derived; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 1
UniProt Protein Name
Leukocyte elastase inhibitor
UniProt Gene Name
SERPINB1
UniProt Synonym Gene Names
ELANH2; MNEI; PI2; LEI; EI; M/NEI; PI-2
UniProt Entry Name
ILEU_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

SERPINB1: Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Belongs to the serpin family. Ov-serpin subfamily.

Chromosomal Location of Human Ortholog: 6p25

Cellular Component: extracellular space; membrane; cytoplasm

Molecular Function: serine-type endopeptidase inhibitor activity

Research Articles on SERPINB1

Similar Products

Product Notes

The SERPINB1 serpinb1 (Catalog #AAA6133687) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SERPINB1 (Leukocyte Elastase Inhibitor, LEI, Monocyte/Neutrophil Elastase Inhibitor, EI, M/NEI, Peptidase Inhibitor 2, PI-2, Serpin B1, ELANH2, MNEI, PI2) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SERPINB1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SERPINB1 serpinb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SERPINB1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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