Mouse anti-Human Collagen Type I Monoclonal Antibody
Collagen Type I (PE)
Reactivity
Human
Applications
ELISA, Immunohistochemistry
Purity
Purified by ammonium sulfate precipitation and chromatography on DEAE-cellulose.
Synonyms
Collagen Type I; Monoclonal Antibody; Collagen Type I (PE); anti-Collagen Type I antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1.BB.748
Specificity
Recognizes both native and heat denatured human collagen type I. No crossreactivity with albumin fractions of human serum or albumins of other animal species. Less than 5% binding to collagens II and III in ELISA, RIA or immunoblotting.
Purity/Purification
Purified by ammonium sulfate precipitation and chromatography on DEAE-cellulose.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-Collagen Type I antibody
ELISA (EIA), Immunohistochemistry (IHC) Frozen
Application Notes
ELISA: Apparent binding constant from titration curves is approximately 0.1nM. Suitable for use in sandwich ELISA for specific determination of collagen I, employed as either a capture antibody or as a second, detecting antibody.
IHC-F: 10-20ug/ml. Formaldehyde fixation is not recommended. Not suitable for use on paraffin embedded sections. Staining of 10um thick cryostat sections fixed with acetone or ethanol. Staining is similar to that for polyclonal antibodies to interstitial collagen I.
Does not work with Western Blot.
Applications are based on unconjugated antibody.
IHC-F: 10-20ug/ml. Formaldehyde fixation is not recommended. Not suitable for use on paraffin embedded sections. Staining of 10um thick cryostat sections fixed with acetone or ethanol. Staining is similar to that for polyclonal antibodies to interstitial collagen I.
Does not work with Western Blot.
Applications are based on unconjugated antibody.
Immunogen
Human Collagen Type I.
Conjugate
PE
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Product Categories/Family for anti-Collagen Type I antibody
References
British Journal of Haematology, 90:757-766, 1995.
Similar Products
Product Notes
The Collagen Type I (Catalog #AAA6125785) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Collagen Type I (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen Type I can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC) Frozen. ELISA: Apparent binding constant from titration curves is approximately 0.1nM. Suitable for use in sandwich ELISA for specific determination of collagen I, employed as either a capture antibody or as a second, detecting antibody. IHC-F: 10-20ug/ml. Formaldehyde fixation is not recommended. Not suitable for use on paraffin embedded sections. Staining of 10um thick cryostat sections fixed with acetone or ethanol. Staining is similar to that for polyclonal antibodies to interstitial collagen I. Does not work with Western Blot. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Collagen Type I for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen Type I, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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