anti-Human Livin Control Peptide Polyclonal Antibody

Anti - Livin Control Peptide (ML-IAP)

Cat. Num. AAA612562

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by immunoaffinity chromatography.
• Synonyms: Livin Control Peptide; Polyclonal Antibody; Anti - Livin Control Peptide (ML-IAP); Livin Control Peptide (ML-IAP); anti-Livin Control Peptide antibody

• Host: Host: Rabbit; Source: Human
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human control peptide has no significant sequence homology with other IAPs. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity.
• Purity/Purification: Purified by immunoaffinity chromatography.
• Form/Format: Affinity Purified; Supplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide, 40% glycerol.

• Applicable Applications for anti-Livin Control Peptide antibody: ELISA, Western Blot
• Application Notes: Suitable for use in ELISA, Western Blotting. Other applications not tested.; Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL. Livin is ~32kD protein (1).; ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.; Optimal dilutions to be determined by the researcher.
• Immunogen: 15-aa peptide from human Livin (1); Designation
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable.

Related Product Information for anti-Livin Control Peptide antibody

Apoptosis or programmed cell death is a fundamental cellular process that is essential for normal tissue development and abnormal growth such as cancer, neurodegeneration, autoimmune diseases, and angiogenesis, etc. Apoptosis is driven by specialized proteases known as caspases (Cysteine Aspartase). The inhibitor-of-apoptosis protein (IAPs) by virtue of inhibiting caspase activity has widespread anti-apoptotic potential family. IAPs are characterized by one or more repeats of a highly conserved ~70 amino acid domain termed the baculoviral IAP repeat (BIR). There are at least five human IAP family members, c-IAP1, c-IAP2, XIAP, NAIP, and survivin. All of the human IAP family members, with the exception of NAIP, have been shown to interact with specific cysteine proteases, or caspases. The caspases are synthesized as inactive zymogen forms which upon apoptotic stimulation are proteolytically processed in a sequential manner into their active heterotetrameric forms. The BIR domain is critical motif for anti-apoptotic activity and interaction with caspases. Many of the IAPs also contain a RING domain near their COOH-termini that is not required for the anti-apoptotic activity.

Product Categories/Family for anti-Livin Control Peptide antibody

Abs to Apoptosis

References

• 1. Kasof GM (2000) JBC, 276, 3238; Lin JH et al (2000) BBRC 279, 820; Vucic D et al (2000) Curr. Biol. 10, 1359

NCBI and Uniprot Product Information

• NCBI GI #: 37182790
• NCBI Official Full Name: LIVIN