Mouse anti-Human, Mouse Cytokeratin 6 Monoclonal Antibody | anti-KRT6 antibody

Cytokeratin 6 (CK6A, CK6B, CK6C, CK6D, CK6E, Keratin Type II Cytoskeletal 6A, Keratin Type II Cytoskeletal 6B, Keratin Type II Cytoskeletal 6C, Keratin Type II Cytoskeletal 6D, Keratin Type II Cytoskeletal 6E, KRT6, KRT6A, KRT6B, KRT6C, KRT6D, KRT6E) (FIT

Cat. Num. AAA6123019

• Gene Names: KRT6A; K6A; K6C; K6D; PC3; CK6A; CK6C; CK6D; KRT6C; KRT6D
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: Cytokeratin 6; Monoclonal Antibody; Cytokeratin 6 (CK6A; CK6B; CK6C; CK6D; CK6E; Keratin Type II Cytoskeletal 6A; Keratin Type II Cytoskeletal 6B; Keratin Type II Cytoskeletal 6C; Keratin Type II Cytoskeletal 6D; Keratin Type II Cytoskeletal 6E; KRT6; KRT6A; KRT6B; KRT6C; KRT6D; KRT6E) (FIT; CK6A; KRT6E; anti-KRT6 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: LHK6
• Specificity: Recognizes Cytokeratin 6. Species Crossreactivity: human and mouse. Others not known.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-KRT6 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Frozen/Paraffin, Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: FC/FACS: 0.5-1ug/million cells; IHC-F/P: 0.5-1.0ug/ml for 30 minutes at RT; IP: 0.5-1ug/500ug protein lysate; IF: 0.5-1ug/ml; WB: 0.5-1.0ug/ml; Applications are based on unconjugated antibody.
• Immunogen: A synthetic peptide of 11 amino acids (GSSTIKYTTTS) from C-terminus of human keratin 6. Cellular Localization: Cytoplasmic
• Conjugate: FITC
• Positive Control: Suprabasal cells of the outer hair follicle root sheath in skin. Basal cell carcinoma
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.; ; FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Categories/Family for anti-KRT6 antibody

Antibodies; Cytokeratin

References

1. Wetzels RH, et. al. American Journal of Pathology, 1991, 138(3):751-63.

NCBI and Uniprot Product Information

• NCBI GI #: 1346344
• NCBI GeneID: 3853
• UniProt Accession #: P02538; P04259; P48668
• Molecular Weight: 564
• NCBI Official Full Name: Keratin, type II cytoskeletal 6A
• NCBI Official Synonym Full Names: keratin 6A
• NCBI Official Symbol: KRT6A
• NCBI Official Synonym Symbols: K6A; K6C; K6D; PC3; CK6A; CK6C; CK6D; KRT6C; KRT6D
• NCBI Protein Information: keratin, type II cytoskeletal 6A; KDAMP; cytokeratin 6A; cytokeratin 6C; cytokeratin 6D; type-II keratin Kb6; keratin, epidermal type II, K6A; kamp-keratin derived antimicrobial peptide
• UniProt Protein Name: Keratin, type II cytoskeletal 6A
• UniProt Gene Name: KRT6A
• UniProt Synonym Gene Names: K6A; KRT6D; CK-6A; CK-6D; K6A
• UniProt Entry Name: K2C6A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. In addition, peptides from the C-terminal region of the protein have antimicrobial activity against bacterial pathogens. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Oct 2014]

Uniprot Description

K6a: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Associates with K16 and/or -17.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: membrane; keratin filament; nucleus

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: wound healing; positive regulation of cell proliferation; morphogenesis of an epithelium; cell differentiation

Disease: Pachyonychia Congenita 3