Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human skin stained with Cytokeratin AE1 antibody LMW (1:200), peroxidase-conjugate and DAB chromogen.)
Mouse Keratin Monoclonal Antibody | anti-KRT1 antibody
Keratin, LMW (Low Molecular Weight Keratin, Type 1 Keratin, Type 1 Cytokeratin, Acidic Keratin, Acidic Cytokeratin, Epithelial Type 1 Keratin) (HRP)
Gene Names
KRT1; K1; CK1; EHK; EHK1; EPPK; KRT1A; NEPPK
Reactivity
Bovine, Canine, Chicken, Human, Monkey, Mouse, Rabbit, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
Keratin; Monoclonal Antibody; LMW (Low Molecular Weight Keratin; Type 1 Keratin; Type 1 Cytokeratin; Acidic Keratin; Acidic Cytokeratin; Epithelial Type 1 Keratin) (HRP); anti-KRT1 antibody
Host
Mouse
Reactivity
Bovine, Canine, Chicken, Human, Monkey, Mouse, Rabbit, Rat
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
AE-1
Specificity
Recognizes human acidic (type 1, epithelial) keratins/cytokeratins. Species Crossreactivity: monkey, bovine, canine, rabbit, mouse, rat, chicken and turtle.
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with horseradish peroxidase (HRP).
Applicable Applications for anti-KRT1 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
WB: 0.5-1ug/ml
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
Human epidermal keratin
Conjugate
HRP
Positive Control
Skin, Squamous cell carcinoma (SCC)
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human skin stained with Cytokeratin AE1 antibody LMW (1:200), peroxidase-conjugate and DAB chromogen.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human skin stained with Cytokeratin AE1 antibody LMW (1:200), peroxidase-conjugate and DAB chromogen.)
Product Categories/Family for anti-KRT1 antibody
References
1. Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8. 2. Tseng SCG et. al. Cell 1982; 30361.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
66kDa
NCBI Official Full Name
keratin, type II cytoskeletal 1
NCBI Official Synonym Full Names
keratin 1
NCBI Official Symbol
KRT1
NCBI Official Synonym Symbols
K1; CK1; EHK; EHK1; EPPK; KRT1A; NEPPK
NCBI Protein Information
keratin, type II cytoskeletal 1
UniProt Protein Name
Keratin, type II cytoskeletal 1
Protein Family
UniProt Gene Name
KRT1
UniProt Synonym Gene Names
KRTA; CK-1; K1
NCBI Description
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
Uniprot Description
May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.
Research Articles on KRT1
Similar Products
Product Notes
The KRT1 krt1 (Catalog #AAA6122301) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Keratin, LMW (Low Molecular Weight Keratin, Type 1 Keratin, Type 1 Cytokeratin, Acidic Keratin, Acidic Cytokeratin, Epithelial Type 1 Keratin) (HRP) reacts with Bovine, Canine, Chicken, Human, Monkey, Mouse, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Keratin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). WB: 0.5-1ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the KRT1 krt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Keratin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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