Western Blot (WB)
(Western blot analysis of NUCL Antibody in 293 cell line lysates (35ug/lane). NUCL (arrow) was detected using the purified Mab.)
Mouse anti-Human NUCL Monoclonal Antibody | anti-NUCL antibody
NUCL (NCL, Nucleolin, Protein C23) (PE)
IHC: 1:50-100
WB: 1:500-1000
IF: 1:10-50
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of NUCL Antibody in 293 cell line lysates (35ug/lane). NUCL (arrow) was detected using the purified Mab.)
Western Blot (WB)
(Western blot analysis of NUCL Antibody in 293 cell line lysates (35ug/lane). NUCL (arrow) was detected using the purified Mab.)
Immunohistochemistry (IHC)
(NUCL Monoclonal Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NUCL Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(NUCL Monoclonal Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NUCL Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of NUCL Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of NUCL Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).)
NCBI and Uniprot Product Information
NCBI Description
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]
Uniprot Description
NCL: the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and preribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-RNA transcription and ribosome assembly.
Protein type: Nuclear receptor co-regulator; DNA-binding; RNA-binding; Nucleolus; Helicase
Chromosomal Location of Human Ortholog: 2q37.1
Cellular Component: nucleoplasm; membrane; nucleolus; cell cortex; nucleus; ribonucleoprotein complex
Molecular Function: protein C-terminus binding; identical protein binding; protein binding; RNA binding; nucleotide binding; telomeric DNA binding
Biological Process: angiogenesis
Research Articles on NUCL
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Product Notes
The NUCL ncl (Catalog #AAA6121729) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NUCL (NCL, Nucleolin, Protein C23) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NUCL can be used in a range of immunoassay formats including, but not limited to, FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). FLISA: 1:1000 IHC: 1:50-100 WB: 1:500-1000 IF: 1:10-50 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NUCL ncl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUCL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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