Goat Hemagglutinin Tag Polyclonal Antibody
Anti - Hemagglutinin Tag (YPYDVPDYA) (HA Tag) (Agarose)
Applications
ELISA
Purity
Purified by immunoaffinity chromatography prior to conjugation.
Synonyms
Hemagglutinin Tag; Polyclonal Antibody; Anti - Hemagglutinin Tag (YPYDVPDYA) (HA Tag) (Agarose); Hemagglutinin Tag (YPYDVPDYA) (HA Tag) (Agarose); anti-Hemagglutinin Tag antibody
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes Hemagglutinin Tag.
Purity/Purification
Purified by immunoaffinity chromatography prior to conjugation.
Form/Format
Affinity Purified
Supplied as a liquid in PBS, pH 7.2. Conjugated to Sepharose.
Supplied as a liquid in PBS, pH 7.2. Conjugated to Sepharose.
Applicable Applications for anti-Hemagglutinin Tag antibody
ELISA
Application Notes
Suitable for use in ELISA. Other applications not tested.
Immunogen
A 9aa peptide sequence from hemagglutinin influenza virus, aa114-122 [YPY DVP DYA] was synthesized and purifed by HPLC.
Preparation and Storage
May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note: We do not recommended adding glycerol as a cryopreservative.
Note: We do not recommended adding glycerol as a cryopreservative.
Related Product Information for anti-Hemagglutinin Tag antibody
Expression of genes in E. coli or yeast or baculovirus offers a convenient system to produce large amounts of recombinant proteins that may otherwise be difficult to isolate from natural cells and tissues. Very often antibodies to these newly identified proteins are not available to study its biochemical properties, monitor protein expression, and purification. In order to circumvent this problem, short pieces of well-defined peptides (Poly-His, DYKDDDDK-epitope or c-myc epitope or HA-tag) or small proteins (bacterial GST, MBP, Thioredoxin, b-Galactosidase, VSV-Glycoprotein etc) are often cloned along with the target gene. Proteins are expressed as fusion proteins. Antibodies to these fusion-tags are already available to monitor fusion protein expression and purification. Therefore, fusion-tags serve as universal tags much like secondary antibodies. Many tags have their own characteristics. Poly-His-fusion proteins (6 x His) can bind to Nickel-Sepharose or Nickel-HRP. GST-fusion proteins can bind to glutathione-Sepharose. Therefore, a high degree of purification of fusion protein can be achieved in just one affinity purification step. Purity of fusion proteins can be followed by Tag-antibodies. Very often, fusion proteins are directly injected into animals to generate antibodies. Some fusion tags can be removed later by treatment with enzymes to generate tag-free recombinant proteins.
Product Categories/Family for anti-Hemagglutinin Tag antibody
References
• Gazin C et al (1984) EMBO J 3, 383-387; Tachibana K et al (1992) Gene, in press.
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Product Notes
The Hemagglutinin Tag (Catalog #AAA612106) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Hemagglutinin Tag can be used in a range of immunoassay formats including, but not limited to, ELISA. Suitable for use in ELISA. Other applications not tested. Researchers should empirically determine the suitability of the Hemagglutinin Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hemagglutinin Tag, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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