Rabbit anti-Human, Mouse b-Amyloid Precursor Protein Polyclonal Antibody

b-Amyloid Precursor Protein

Cat. Num. AAA612104

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: b-Amyloid Precursor Protein; Polyclonal Antibody; Anti -b-Amyloid Precursor Protein; anti-b-Amyloid Precursor Protein antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Amyloid Precursor Protein. Reactivity has been confirmed with APLP2 (amyloid precursor-like protein 2) -/- knock-out and wildtype mouse brain homogenates and APP695 C99 (C-terminal fragment)-transfected HEK293 cell lysates by Western Blot. Negative control tests with APP -/- knock-out and APP/APLP2 double knock-out mice have confirmed the specificity of this antibody; neither of the target bands were identified in these test samples. Reactivity has also been confirmed with human Alzheimer's brain tissue by immunohistochemistry. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-b-Amyloid Precursor Protein antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.; Dilution: Western Blot (Mouse): 1-3ug/ml. Detects a band representing the full-length protein at ~ 100kD, and a band at ~ 10-12kD representing the C99 C-terminal cleavage fragment. A major non-specific band has been observed at ~50kD in mouse brain homogenates.; Immunoprecipitation (Mouse): 7ug/reaction; Immunohistochemistry (FFPE, Human): 1-2ug/ml. Digestion with trypsin prior to staining is required.
• Immunogen: Synthetic peptide derived from the C-terminal region of the human and mouse amyloid-beta precursor protein (APP695).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-b-Amyloid Precursor Protein antibody

Amyloid-beta precursor protein (APP) is the source of the amyloid-beta (Ab) peptide found in neuritic plaques of Alzheimer's disease (AD) patients. APP is aglycosylated transmembrane protein with a long extracellular N-terminal domain, a short intracellular C-terminal domain, and an Abeta-segment of intermediate length shared between the intra- and extracellular regions. APP is cleaved by alpha-secretase immediately before or after reaching the cell surface, releasing the nonamyloidogenic secreted form of the N-terminal soluble APP fragment (sAPPa). APP molecules that are not cleaved by alpha-secretase are internalized, and subjected to beta-site cleavage by beta-secretase, leaving behind a membrane-bound C-terminal stub. This terminal stub is the substrate for gamma-secretase, which cleaves the molecule at gamma-site(s) to release the 40aa, 42aa, or 43aa -long Ab peptides.

Product Categories/Family for anti-b-Amyloid Precursor Protein antibody

Antibodies; Abs to Amyloid