Goat anti-Human UBE2V1 Polyclonal Antibody | anti-UBE2V1 antibody

UBE2V1 (TMEM189-UBE2V1 readthrough transcript, CROC1B, KUA-UEV)

Cat. Num. AAA611592

• Gene Names: UBE2V1; CIR1; UEV1; CROC1; UBE2V; UEV-1; UEV1A; CROC-1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: UBE2V1; Polyclonal Antibody; UBE2V1 (TMEM189-UBE2V1 readthrough transcript; CROC1B; KUA-UEV); Anti -UBE2V1 (TMEM189-UBE2V1 readthrough transcript; anti-UBE2V1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: This antibody is expected to recognize human isoform b only (NP_003340).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-UBE2V1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA, Western Blot., Western blot: Approx 27kD band seen in K562 lysate. Recommended for use at 2-4ug/ml., Staining (3ug/ml) of K562 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Optimal dilutions to be determined by the researcher.
• Immunogen: AYKFRTHSPEALE-C, N Terminus
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-UBE2V1 antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 56203164
• NCBI GeneID: 7335
• UniProt Accession #: Q13404; Q13403; Q13532; Q5TGE3; Q96H34; Q9GZT0; Q9GZW1; Q9H4J3; Q9H4J4; Q9UKL1; Q9UM48; E1P629; Q5TGE1
• Molecular Weight: 16,495 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 variant 1
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2 variant 1
• NCBI Official Symbol: UBE2V1
• NCBI Official Synonym Symbols: CIR1; UEV1; CROC1; UBE2V; UEV-1; UEV1A; CROC-1
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 variant 1; OTTHUMP00000031795; OTTHUMP00000031796; OTTHUMP00000031801; OTTHUMP00000031805; OTTHUMP00000039980; DNA-binding protein; TRAF6-regulated IKK activator 1 beta Uev1A
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 variant 1
• UniProt Gene Name: UBE2V1
• UniProt Synonym Gene Names: CROC1; UBE2V; UEV1
• UniProt Entry Name: UB2V1_HUMAN

NCBI Description

Ubiquitin-conjugating E2 enzyme variant proteins constitute a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. The protein encoded by this gene is located in the nucleus and can cause transcriptional activation of the human FOS proto-oncogene. It is thought to be involved in the control of differentiation by altering cell cycle behavior. Multiple alternatively spliced transcripts encoding different isoforms have been described for this gene. A pseudogene has been identified which is also located on chromosome 20. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (Kua-UEV), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq]

Uniprot Description

UBE2V1: Has no ubiquitin ligase activity on its own. The UBE2V1- UBE2N heterodimer catalyzes the synthesis of non-canonical poly- ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Belongs to the ubiquitin-conjugating enzyme family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: protein complex; ubiquitin conjugating enzyme complex; cytoplasm; nucleus; cytosol; ubiquitin ligase complex

Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin conjugating enzyme binding; ubiquitin protein ligase binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; protein polyubiquitination; error-free postreplication DNA repair; postreplication repair; positive regulation of transcription, DNA-dependent; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; regulation of DNA repair; regulation of transcription, DNA-dependent; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; cell differentiation; toll-like receptor 4 signaling pathway