Rabbit anti-Human, Mouse MDA5 Polyclonal Antibody | anti-IFIH1 antibody

MDA5, CT (Melanoma Differentiation-Associated Protein 5, Interferon-Induced with Helicase C Domain Protein 1, IFIH1)

Cat. Num. AAA611545

• Gene Names: IFIH1; Hlcd; MDA5; MDA-5; IDDM19; MGC133047
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: MDA5; Polyclonal Antibody; CT (Melanoma Differentiation-Associated Protein 5; Interferon-Induced with Helicase C Domain Protein 1; IFIH1); Anti -MDA5; anti-IFIH1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MDA5. Species Crossreactivity: Mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-IFIH1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1-2ug/ml. See M2772-76A for control peptide.
• Immunogen: 16 amino acid peptide from near the carboxy terminus of human MDA5 (GenBank accession no. NP_071451)
• Positive Control: Daudi cell lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4ug/ml.)

Related Product Information for anti-IFIH1 antibody

The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses (1,2). Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family (3), consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF- B (4). MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity (5).

Product Categories/Family for anti-IFIH1 antibody

Antibodies; Abs to DEAD Box Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 11344594
• NCBI GeneID: 64135
• UniProt Accession #: Q9BYX4; Q2NKL6; Q6DC96; Q86X56; Q96MX8; Q9H3G6; Q53TB6; Q53TP4
• Molecular Weight: 116,689 Da
• NCBI Official Full Name: melanoma differentiation associated protein-5
• NCBI Official Synonym Full Names: interferon induced with helicase C domain 1
• NCBI Official Symbol: IFIH1
• NCBI Official Synonym Symbols: Hlcd; MDA5; MDA-5; IDDM19; MGC133047
• NCBI Protein Information: interferon-induced helicase C domain-containing protein 1; helicard; OTTHUMP00000162935; CADM-140 autoantigen; helicase with 2 CARD domains; RNA helicase-DEAD box protein 116; murabutide down-regulated protein; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; melanoma differentiation associated protein-5; melanoma differentiation-associated protein 5; interferon-induced with helicase C domain protein 1; clinically amyopathic dermatomyositis autoantigen 140 kDa
• UniProt Protein Name: Interferon-induced helicase C domain-containing protein 1
• Protein Family: Interferon-induced helicase C domain-containing protein
• UniProt Gene Name: IFIH1
• UniProt Synonym Gene Names: MDA5; RH116
• UniProt Entry Name: IFIH1_HUMAN

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. [provided by RefSeq]

Uniprot Description

IFIH1: Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O- methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV) and mengo encephalomyocarditis virus (ENMG). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines. Monomer in the absence of ligands and homodimerizes in the presence of dsRNA ligands. Can assemble into helical or linear polymeric filaments on long dsRNA. Interacts with MAVS/IPS1. Interacts with V protein of Simian virus 5, Human parainfluenza virus 2, Mumps virus, Sendai virus and Hendra virus. Binding to paramyxoviruses V proteins prevents IFN-beta induction, and the further establishment of an antiviral state. Interacts with PCBP2. Interacts with NLRC5. Interacts with PIAS2-beta. Interacts with DDX60. By interferon (IFN) and TNF. Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung. Belongs to the helicase family. RLR subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Helicase; EC 3.6.4.13

Chromosomal Location of Human Ortholog: 2q24

Cellular Component: nucleus; cytosol

Molecular Function: ribonucleoprotein binding; protein binding; single-stranded RNA binding; DNA binding; zinc ion binding; double-stranded RNA binding; helicase activity; ATP binding

Biological Process: regulation of apoptosis; protein sumoylation; viral reproduction; detection of virus; response to virus; positive regulation of interferon-beta production; innate immune response; negative regulation of interferon type I production; positive regulation of interferon-alpha production

Disease: Aicardi-goutieres Syndrome 7; Singleton-merten Syndrome 1