Rabbit Leptin Receptor Gene Related Protein Polyclonal Antibody | anti-LEPROT antibody

Leptin Receptor Gene Related Protein (OBGRP)

Cat. Num. AAA611217

• Gene Names: LEPROT; LEPR; VPS55; OBRGRP; OB-RGRP; FLJ37482; FLJ90360
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Leptin Receptor Gene Related Protein; Polyclonal Antibody; Leptin Receptor Gene Related Protein (OBGRP); Anti -Leptin Receptor Gene Related Protein (OBGRP); anti-LEPROT antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human OB-RGRP. Species Crossreactivity: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA.

• Applicable Applications for anti-LEPROT antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1-10ug/ml; ELISA: 1:10,000-50,000; 10-100ng of control peptide/well.
• Immunogen: A synthetic peptide corresponding to 15aa within the first putative intracellular domain of human OB-RGRP, conjugated to KLH. Species Sequence Homology: human LEPROTL1, 53%
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-LEPROT antibody

Obesity, a common nutritional disorder, is associated with diabetes, hypertension, hyperlipidemia, cancer and many other health related problems. At least five genes, Obese (ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub) have been linked to obesity. Obese gene encodes an adipocyte-tissue derived secreted protein Ob protein/Leptin (167 amino acid, ~16kD) that controls body weight homeostasis. Leptin mediates its effects via the Leptin receptor or Obese receptors (OBR or LR) that is expressed in several tissues including hypothalamus. The Ob-R has at least 6 alternatively spliced forms (OBRa-f or LRa-f) that contain a common extracellular domain. The OBRa represents the initially identified mouse Ob-R (short form, 894aa). OBRa, -c, -d, and -f differ in sequence after Lys889 and have short (30-40 aa) cytoplasmic extension Mouse Ob-Rb (long form) displays ~78% homology to the human Ob-R (long form, 1165aa). OBRb has ~300 aa intracellular tail. Expression of Ob-Rb and other forms have been detected in hypothalamus and other tissues. OBRe lacks the transmembrane domain. The soluble Ob-Re is found in adipose tissues, hypothalamus, heart, and testes. Ob-R is abnormally spliced in db/db mice (truncation of cytoplasmic domain) that are important for leptin signaling.

Product Categories/Family for anti-LEPROT antibody

Antibodies; Abs to Obesity Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 8923785
• NCBI GeneID: 54741
• NCBI Accession #: NP_059996.1
• NCBI GenBank Nucleotide #: NM_017526.4
• UniProt Accession #: O15243; Q6FHL5
• Molecular Weight: 14,254 Da
• NCBI Official Full Name: leptin receptor gene-related protein isoform 1
• NCBI Official Synonym Full Names: leptin receptor overlapping transcript
• NCBI Official Symbol: LEPROT
• NCBI Official Synonym Symbols: LEPR; VPS55; OBRGRP; OB-RGRP; FLJ37482; FLJ90360
• NCBI Protein Information: leptin receptor gene-related protein; OTTHUMP00000010632; OB-R gene-related protein; leptin receptor gene related protein; DnaJ (Hsp40) homolog, subfamily C, member 6; leptin receptor overlapping transcript protein
• UniProt Protein Name: Leptin receptor gene-related protein
• Protein Family: Leptin receptor gene-related protein
• UniProt Gene Name: LEPROT
• UniProt Synonym Gene Names: LEPR; OBR
• UniProt Entry Name: OBRG_HUMAN

NCBI Description

LEPROT is associated with the Golgi complex and endosomes and has a role in cell surface expression of growth hormone receptor (GHR; MIM 600946) and leptin receptor (OBR, or LEPR; MIM 601007), thereby altering receptor-mediated cell signaling (Couturier et al., 2007 [PubMed 18042720]; Touvier et al., 2009 [PubMed 19907080]).[supplied by OMIM]

Uniprot Description

LEPROT: Negatively regulates leptin receptor (LEPR) cell surface expression, and thus decreases response to leptin. Negatively regulates growth hormone (GH) receptor cell surface expression in liver. May play a role in liver resistance to GH during periods of reduced nutrient availability. Belongs to the OB-RGRP/VPS55 family.

Protein type: Membrane protein, integral; Receptor, misc.; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: Golgi membrane; Golgi apparatus; integral to membrane; endosome membrane; endosome

Molecular Function: receptor binding

Biological Process: negative regulation of JAK-STAT cascade