Rabbit Pyruvate Kinase, Tumor Type M2 Polyclonal Antibody | anti-PKM2 antibody

Pyruvate Kinase, Tumor Type M2 (PKM2)

Cat. Num. AAA611179

• Gene Names: PKM2; PK3; PKM; TCB; OIP3; CTHBP; THBP1; MGC3932
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Synonyms: Pyruvate Kinase; Tumor Type M2; Polyclonal Antibody; Tumor Type M2 (PKM2); Anti -Pyruvate Kinase; anti-PKM2 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of total human PKM2 protein at 60kD. Species Crossreactivity: monkey, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-PKM2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:1000; Immunohistochemistry (Paraffin): 1:100; Using a citrate unmasking buffer.; Immunohistochemistry (Frozen): 1:100; Using a 3% formaldehyde fixative.
• Immunogen: Synthetic peptide corresponding to the sequence of human PKM2 conjugated to KLH. Molecular Weight: 60kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of extracts from various cell lines using MBS611179.)

Western Blot (WB)

(Western Blot analysis of extracts from various tissue and cell lysates using MBS611179.)

Testing Data

Testing Data

Related Product Information for anti-PKM2 antibody

Pyruvate kinase, a glycolytic enzyme, catalyzes the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues (1). The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development (1). Studies found that cancer cells exclusively express PKM2 (1-3). PKM2 is shown to be essential for aerobic glycolysis in tumors (Warburg effect) (1). When the M2 isoform is switched to the M1 isoform, aerobic glycolysis is reduced and oxidative phosphorylation is increased in cancer cells (1). These cells also show decreased tumorigenicity in mouse xenografts (1). Recent studies show that the oncogenic forms of FGFR1 directly phosphorylate Tyr105 of PKM2 and thereby inhibit the formation of active tetrameric PKM2 (4). A PKM2 mutant found in cancer cells, in which Tyr105 is replaced by phenylalanine, leads to reduced cell proliferation in hypoxia and tumor growth in xenografts in nude mice (4). These findings suggest that the phosphorylation at Tyr105 is a critical switch for the metabolism in cancer cells that promotes tumor growth (4).

Product Categories/Family for anti-PKM2 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 33286418
• NCBI GeneID: 5315
• NCBI Accession #: NP_002645.3
• NCBI GenBank Nucleotide #: NM_002654.4
• UniProt Accession #: P14618; P14786; Q53GK4; Q96E76; Q9BWB5; Q9UCV6; Q9UPF2; A6NFK3; B2R5N8; B3KRY0; B4DFX8; Q504U3; Q8WUW7; Q9NYI7; Q9UK31; Q9UKK4
• Molecular Weight: 57,937 Da
• NCBI Official Full Name: pyruvate kinase isozymes M1/M2 isoform a
• NCBI Official Synonym Full Names: pyruvate kinase, muscle
• NCBI Official Symbol: PKM2
• NCBI Official Synonym Symbols: PK3; PKM; TCB; OIP3; CTHBP; THBP1; MGC3932
• NCBI Protein Information: pyruvate kinase isozymes M1/M2; p58; OIP-3; tumor M2-PK; PK, muscle type; pyruvate kinase 2/3; OPA-interacting protein 3; pyruvate kinase muscle isozyme; thyroid hormone-binding protein 1; cytosolic thyroid hormone-binding protein; thyroid hormone-binding protein, cytosolic
• UniProt Protein Name: Pyruvate kinase isozymes M1/M2
• UniProt Gene Name: PKM2
• UniProt Synonym Gene Names: OIP3; PK2; PK3; PKM
• UniProt Entry Name: KPYM_HUMAN

NCBI Description

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq]

Uniprot Description

PKM2: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. Overexpressed in a variety of cancers. Interacts with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. Binds Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogens is. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms of PKM2 have been reported.

Protein type: EC 2.7.1.40; Carbohydrate Metabolism - pyruvate; Nucleotide Metabolism - purine; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; Mitochondrial

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: extracellular matrix; mitochondrion; cytoplasm; plasma membrane; cytosol; nucleus; vesicle; cilium

Molecular Function: potassium ion binding; protein binding; magnesium ion binding; pyruvate kinase activity; ATP binding

Biological Process: glycolysis; carbohydrate metabolic process; programmed cell death; glucose metabolic process; pathogenesis; phosphorylation