Rabbit anti-Human Melanin Concentrating Hormone Receptor1 Polyclonal Antibody

Melanin Concentrating Hormone Receptor1 (MCHR1)

Cat. Num. AAA610802

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Melanin Concentrating Hormone Receptor1; Polyclonal Antibody; Melanin Concentrating Hormone Receptor1 (MCHR1); Anti -Melanin Concentrating Hormone Receptor1 (MCHR1); anti-Melanin Concentrating Hormone Receptor1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MCHR1. No significant sequence homology is seen with MCHR2 or with other GPCRs. Species Sequence Homology
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.05% sodium azide, before the addition of glycerol to 40%.

• Applicable Applications for anti-Melanin Concentrating Hormone Receptor1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1-10ug/ml using ECL.; ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
• Immunogen: Synthetic peptide (16aa) near the cytoplasmic C-terminus of human MCHR/GPR24/SLC-1 (KLH coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Melanin Concentrating Hormone Receptor1 antibody

The cyclic neuropeptide, Melanin-Concentrating Hormone (MCH), plays an important role in food intake and energy balance. MCH stimulates feeding. It is up regulated in obese mice and during fasting. Animals lacking MCH eat less and are leaner. A receptor for MCH (MCHR) has been cloned and characterized. Human MCH receptor is a 402aa membrane protein. It is also described as an orphan G-protein coupled receptor SLC-1 or GPR24, somatostatin receptor-like protein. It has 7 transmembrane domains, a Dry motif at the boundary of TM3 and the 2nd intracellular loop, a consensus site for N-linked glycosylation at the N-terminus. MCHR is expressed in ventromedial and dorsomedial nuclei of the hypothalamus. An orphan G-protein coupled receptor (SLC-1, GPR24) has been identified as the receptor of MCH. MCH receptor (MCH1R; human 402aa, rat 353aa) is predicted to contain 7 transmembrane domains, a feature typical of G-protein coupled receptors. Moderate levels of MCHR are also found in eye and skeletal muscle, tongue and pituitary. MCHR binds MCH with sub-nanomolar affinity. It is stimulated by MCH to mobilize intracellular Ca2+ and reduce forskolin-elevated cAMP levels. A second human MCH receptor (MCH2R) has been cloned and characterized. MCH2R gene encodes a 340aa protein with 38% identity with MCH1R. It has the basic characteristics of GPCR and belongs to class 1 (rhodopsin-like) GPCR superfamily. MCH2R transfected cells responds to MCH with an increase in intracellular Ca2+ levels and increased cellular extrusion of protons. MCH2R is mainly expressed in the brain.

Product Categories/Family for anti-Melanin Concentrating Hormone Receptor1 antibody

Antibodies; Abs to Receptors