Rabbit anti-Mouse Ptx3, PiTX3 Polyclonal Antibody | anti-PITX3 antibody

Ptx3, PiTX3 (Homeodomain Transcription Factor-3)

Cat. Num. AAA610568

• Gene Names: PITX3; PTX3; CTPP4; MGC12766
• Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Ptx3; PiTX3; Polyclonal Antibody; PiTX3 (Homeodomain Transcription Factor-3); Anti -Ptx3; anti-PITX3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse PiTX3 protein. Reactivity has been confirmed with fetal mouse brain homogenate.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-PITX3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.; Dilution: Immunofluorescence: 0.7-2ug/ml; Western Blot: 1-3ug/ml. Identifies a single band at ~32kD. Bands of unknown origin are observed at ~40 and 50kD.; Immunohistochemistry: 0.7-2ug/ml. For optimal results, fix the mouse brain in 4% paraformaldehyde overnight.
• Immunogen: Synthetic peptide derived from the N-terminal region of the mouse PiTX3 protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-PITX3 antibody

The paired-like homeodomain transcription factor-3 (PiTX3) is a member of the homeodomain family of DNA binding proteins known as the RIEG/PITX homeobox gene family. The PiTX (PTX) family of homeobox transcription factors includes PiTX1, PiTX2 and PiTX3. PiTX3 is associated with early ocular development and is later expressed in other organs and limbs. PiTX3 is expressed in the eyes (specifically the developing lens), and has a highly restricted expression pattern in the brain. PiTX3 expression in the midbrain starts just before terminal differentiation of dopaminergic neurons (DA). PiTX3 can act on the promoter of the Tyrosine hydroxylase (TH) gene in a cell-type dependent fashion, suggesting that PiTX3 contributes to the regulation of TH expression in mesDA.

Product Categories/Family for anti-PITX3 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4826912
• NCBI GeneID: 5309
• NCBI Accession #: NP_005020.1
• NCBI GenBank Nucleotide #: NM_005029.3
• UniProt Accession #: O75364; Q5VZL2
• Molecular Weight: 31,832 Da
• NCBI Official Full Name: pituitary homeobox 3
• NCBI Official Synonym Full Names: paired-like homeodomain 3
• NCBI Official Symbol: PITX3
• NCBI Official Synonym Symbols: PTX3; CTPP4; MGC12766
• NCBI Protein Information: pituitary homeobox 3; OTTHUMP00000020362; homeobox protein PITX3
• UniProt Protein Name: Pituitary homeobox 3
• Protein Family: Pituitary homeobox
• UniProt Gene Name: PITX3
• UniProt Synonym Gene Names: PTX3
• UniProt Entry Name: PITX3_HUMAN

NCBI Description

This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family act as transcription factors. This protein is involved in lens formation during eye development. Mutations of this gene have been associated with anterior segment mesenchymal dysgenesis and congenital cataracts. [provided by RefSeq]

Uniprot Description

PITX3: Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. In addition to its importance during development, it also has roles in the long-term survival and maintenance of the mdDA neurons. Activates NR4A2/NURR1- mediated transcription of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Acts by decreasing the interaction of NR4A2/NURR1 with the corepressor NCOR2/SMRT which acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Essential for the normal lens development and differentiation. Plays a critical role in the maintenance of mitotic activity of lens epithelial cells, fiber cell differentiation and in the control of the temporal and spatial activation of fiber cell-specific crystallins. Positively regulates FOXE3 expression and negatively regulates PROX1 in the anterior lens epithelium, preventing activation of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 and thus maintains lens epithelial cells in cell cycle. Defects in PITX3 are a cause of cataract autosomal dominant (ADC). Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Cataract is the most common treatable cause of visual disability in childhood. Defects in PITX3 are a cause of anterior segment mesenchymal dysgenesis (ASMD); also known as anterior segment ocular dysgenesis (ASOD). ASMD consists of a range of developmental defects in structures at the front of the eye, resulting from abnormal migration or differentiation of the neural crest derived mesenchymal cells that give rise to the cornea, iris, and other components of the anterior chamber during eye development. Mature anterior segment anomalies are associated with an increased risk of glaucoma and corneal opacity. Conditions falling within the phenotypic spectrum include aniridia, posterior embryotoxon, Axenfeld anomaly, Reiger anomaly/syndrome, Peters anomaly, and iridogoniodysgenesis. Defects in PITX3 are the cause of cataract posterior polar type 4 (CTPP4). A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity. Some patients affected by cataract posterior polar type 4 can present a severe phenotype including microphthalmia and neurological dysfunction. Belongs to the paired homeobox family. Bicoid subfamily.

Protein type: Cell development/differentiation; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 10q24.32

Cellular Component: nucleus

Biological Process: lens development in camera-type eye; organ morphogenesis; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; midbrain development; neuron development; positive regulation of transcription from RNA polymerase II promoter; lens morphogenesis in camera-type eye; locomotory behavior

Disease: Anterior Segment Mesenchymal Dysgenesis; Cataract 11, Multiple Types