Rabbit ILP-interacting protein Polyclonal Antibody | anti-STRADB antibody

ILP-interacting protein (ILPIP, ALSCR2, PAPK)

Cat. Num. AAA610343

• Gene Names: STRADB; PAPK; ILPIP; ILPIPA; ALS2CR2; CALS-21; PRO1038; MGC102916
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ILP-interacting protein; Polyclonal Antibody; ILP-interacting protein (ILPIP; ALSCR2; PAPK); Anti -ILP-interacting protein (ILPIP; anti-STRADB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ILPIP. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.

• Applicable Applications for anti-STRADB antibody: Western Blot (WB)
• Application Notes: Can be used for the detection of ILPIP by Western Blot at 1 to 2ug/ml. Human brain cell lysate can be used as positive control and a 34kD band can be detected.
• Immunogen: Synthetic peptide corresponding to amino acids near the center of human ILPIP (Genbank accession NP_061041)
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of ILPIP in human brain lysate with anti-ILPIP (IN) at 1 (lane A) and 2 ug/ml (lane B), respectively.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of MBS610343.)

Related Product Information for anti-STRADB antibody

ILPIP (ILP-interacting protein) has recently been shown to interact with XIAP, a member of the IAP (Inhibitor of Apoptosis) protein family (1). Together, these two proteins synergistically activate the TAK1/JNK1 signal transduction pathway, which acts to protect against the interleukin-1b converting enzyme of Fas-induced apotosis (1,2). This protein was independently isolated and characterized as polyploidy-associated protein kinase (PAPK), a member of the Ste20/germinal center kinase family that modulates cytoskeletal organization and cell survival (3), suggesting that its protective role may be a function of its kinase activity. Although two mRNA sequences have been isolated, only the shorter isoform has been observed.

Product Categories/Family for anti-STRADB antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 20384952
• NCBI GeneID: 55437
• UniProt Accession #: Q9C0K7; Q5BKY7; Q9P1L0
• Molecular Weight: 47,026 Da
• NCBI Official Full Name: ILP-interacting protein ILPIPA
• NCBI Official Synonym Full Names: STE20-related kinase adaptor beta
• NCBI Official Symbol: STRADB
• NCBI Official Synonym Symbols: PAPK; ILPIP; ILPIPA; ALS2CR2; CALS-21; PRO1038; MGC102916
• NCBI Protein Information: STE20-related kinase adapter protein beta; STRAD beta; OTTHUMP00000163729; OTTHUMP00000206592; pseudokinase ALS2CR2; ILP-interacting protein ILPIPA; amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2; amyotrophic lateral sclerosis 2 chromosomal region candidate gene 2 protein
• UniProt Protein Name: STE20-related kinase adapter protein beta
• Protein Family: STE20-related kinase adapter protein
• UniProt Gene Name: STRADB
• UniProt Synonym Gene Names: ALS2CR2; ILPIP
• UniProt Entry Name: STRAB_HUMAN

NCBI Description

This gene encodes a protein that belongs to the serine/threonine protein kinase STE20 subfamily. One of the active site residues in the protein kinase domain of this protein is altered, and it is thus a pseudokinase. This protein is a component of a complex involved in the activation of serine/threonine kinase 11, a master kinase that regulates cell polarity and energy-generating metabolism. This complex regulates the relocation of this kinase from the nucleus to the cytoplasm, and it is essential for G1 cell cycle arrest mediated by this kinase. The protein encoded by this gene can also interact with the X chromosome-linked inhibitor of apoptosis protein, and this interaction enhances the anti-apoptotic activity of this protein via the JNK1 signal transduction pathway. Two pseudogenes, located on chromosomes 1 and 7, have been found for this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

STLK6: Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; STE group; STE20 family; STLK subfamily

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: protein binding; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity

Biological Process: insulin receptor signaling pathway; cell morphogenesis; activation of protein kinase activity; JNK cascade; mitotic cell cycle; protein export from nucleus; cell cycle arrest; protein amino acid phosphorylation