Rabbit anti-Human KCC4 Polyclonal Antibody | anti-SLC12A7 antibody
KCC4 (K, Cl Cotransporter 4)
Gene Names
SLC12A7; KCC4; DKFZp434F076
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
KCC4; Polyclonal Antibody; KCC4 (K; Cl Cotransporter 4); Anti -KCC4 (K; anti-SLC12A7 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human KCC4. Species Sequence Homology: mouse (89%). No significant sequence homology was found with other KCCs or CCC.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
Applicable Applications for anti-SLC12A7 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot and ELISA.
Dilution: Western Blot: 1-10ug/ml using Chemiluminescence technique.
ELISA: 1:10,000-1:100,000; using 50-100ng of control peptide/well (K0135-01B).
Dilution: Western Blot: 1-10ug/ml using Chemiluminescence technique.
ELISA: 1:10,000-1:100,000; using 50-100ng of control peptide/well (K0135-01B).
Immunogen
19aa peptide of human KCC4 (KLH). Epitope: ~C-terminus, Cytoplasmic.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-SLC12A7 antibody
Chloride is a critical component of all living cells. It is also the single most dominant diffusible anion inside of most cells-the others are mostly impermeable organic anions. Since cytoplasmic electroneutrality is maintained under normal physiological environment, changes in cellular chloride level is accompanied by total cell solute content. The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane.
Product Categories/Family for anti-SLC12A7 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
119,106 Da
NCBI Official Full Name
K-Cl cotransporter KCC4
NCBI Official Synonym Full Names
solute carrier family 12 (potassium/chloride transporters), member 7
NCBI Official Symbol
SLC12A7
NCBI Official Synonym Symbols
KCC4; DKFZp434F076
NCBI Protein Information
solute carrier family 12 member 7; OTTHUMP00000220873; K-Cl cotransporter 4; potassium/chloride transporter KCC4; electroneutral potassium-chloride cotransporter 4
UniProt Protein Name
Solute carrier family 12 member 7
Protein Family
UniProt Gene Name
SLC12A7
UniProt Synonym Gene Names
KCC4
UniProt Entry Name
S12A7_HUMAN
Uniprot Description
KCC4: Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May mediate K(+) uptake into Deiters' cells in the cochlea and contribute to K(+) recycling in the inner ear. Important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti. May be required for basolateral Cl(-) extrusion in the kidney and contribute to renal acidification. Belongs to the SLC12A transporter family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral; Transporter
Chromosomal Location of Human Ortholog: 5p15
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: protein kinase binding; potassium:chloride symporter activity
Biological Process: transport; ion transport; cell volume homeostasis; transmembrane transport; potassium ion transport
Research Articles on SLC12A7
Similar Products
Product Notes
The SLC12A7 slc12a7 (Catalog #AAA610293) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KCC4 (K, Cl Cotransporter 4) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KCC4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot and ELISA. Dilution: Western Blot: 1-10ug/ml using Chemiluminescence technique. ELISA: 1:10,000-1:100,000; using 50-100ng of control peptide/well (K0135-01B). Researchers should empirically determine the suitability of the SLC12A7 slc12a7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KCC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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